In order to observe cells at work, researchers have to bypass a physical law. One of the fastest techniques to overcome the resolution limit of classical light microscopy is high-resolution structured illumination microscopy. It makes visible details that are about a hundred nanometres in s ... more
Starship Enterprise concept: Optical tractor beam traps bacteria
High resolution cell imaging
Up to now, if scientists wanted to study blood cells, algae, or bacteria under the microscope, they had to mount these cells on a substrate such as a glass slide. Physicists at Bielefeld and Frankfurt Universities have developed a method that traps biological cells with a laser beam enabling them to study them at very high resolutions. In science fiction books and films, the principle is known as the 'tractor beam'. Using this procedure, the physicists have obtained superresolution images of the DNA in single bacteria.
One of the problems facing researchers who want to examine biological cells microscopically is that any preparatory treatment will change the cells. Many bacteria prefer to be able to swim freely in solution. Blood cells are similar: They are continuously in rapid flow, and do not remain on surfaces. Indeed, if they adhere to a surface, this changes their structure and they die.
'Our new method enables us to take cells that cannot be anchored on surfaces and then use an optical trap to study them at a very high resolution. The cells are held in place by a kind of optical tractor beam. The principle underlying this laser beam is similar to the concept to be found in the television series "Star Trek",' says Professor Dr. Thomas Huser. He is the head of the Biomolecular Photonics Research Group in the Faculty of Physics. 'What's special is that the samples are not only immobilized without a substrate but can also be turned and rotated. The laser beam functions as an extended hand for making microscopically small adjustments.'
The Bielefeld physicists have further developed the procedure for use in superresolution fluorescence microscopy. This is considered to be a key technology in biology and biomedicine because it delivers the first way to study biological processes in living cells at a high scale -- something that was previously only possible with electron microscopy. To obtain images with such microscopes, researchers add fluorescent probes to the cells they wish to study, and these will then light up when a laser beam is directed towards them. A sensor can then be used to record this fluorescent radiation so that researchers can even gain three-dimensional images of the cells.
In their new method, the Bielefeld researchers use a second laser beam as an optical trap so that the cells float under the microscope and can be moved at will. 'The laser beam is very intensive but invisible to the naked eye because it uses infrared light,' says Robin Diekmann, a member of the Biomolecular Photonics Research Group. 'When this laser beam is directed towards a cell, forces develop within the cell that hold it within the focus of the beam,' says Diekmann. Using their new method, the Bielefeld physicists have succeeded in holding and rotating bacterial cells in such a way that they can obtain images of the cells from several sides. Thanks to the rotation, the researchers can study the three-dimensional structure of the DNA at a resolution of circa 0.0001 millimetres.
Professor Huser and his team want to further modify the method so that it will enable them to observe the interplay between living cells. They would then be able to study, for example, how germs penetrate cells.
To develop the new methods, the Bielefeld scientists are working together with Prof. Dr. Mike Heilemann and Christoph Spahn from the Johann Wolfgang Goethe University of Frankfurt am Main.
This information can be used to produce images with a resolution of about 20 to 30 nanometres, and thereby ten times that of conventional light microscopy. Until now, this method has required the use of expensive special instruments. Bielefeld University and the University of Tromsø have fi ... more
Optical imaging of highly motile cells or cells in suspension, such as bacterial systems, yeast cells, and immune cells, is a challenging task, in many cases it is just not possible. The human immunodeficiency virus (HIV), for example, can spread very efficiently from an infected T cell to ... more
A team from Research Unit 2251 of the German Research Foundation led by Goethe University has shed light on the structure of an enzyme important in the metabolism of the pathogenic bacterium Acinetobacter baumannii. The enzyme “MtlD” is critical for the bacterium’s synthesis of the sugar al ... more
Catalysts with a metal-nitrogen bond can transfer nitrogen to organic molecules. In this process short-lived molecular species are formed, whose properties critically determine the course of the reaction and product formation. The key compound in a catalytic nitrogen-atom transfer reaction ... more
An interdisciplinary team from Frankfurt and Jena has developed a kind of bait with which to fish protein complexes out of mixtures. Thanks to this “bait”, the desired protein is available much faster for further examination in the electron microscope. The research team has christened this ... more
- 1Smartphone-powered microchip for at-home medical diagnostic testing developed
- 2A new method for atomic-resolution electron microscopes
- 3Endress+Hauser bundles analysis expertise
- 4Measuring the ‘wettability’ of graphene and other 2D materials
- 5Olympus Makes it EVIDENT
- 6Why are neuron axons long and spindly?
- 7analytica 2022: Analytics that benefit consumer and environmental protection
- 8Seeing more deeply into nanomaterials
- 9Bruker and TOFWERK Form Strategic Partnership
- 10Researchers demonstrate label-free super-resolution microscopy