Guide to the purification of antibodies using ion exchange chromatography

In biopharmaceutical manufacturing, efficient and sustainable purification processes are becoming increasingly important. This Whitepaper demonstrates how ion exchange chromatography (IEX) can be purposefully applied to optimise antibody purification. It is aimed at those who wish to critically evaluate existing purification strategies and adapt them to meet new demands – both in terms of product quality and in relation to economic and environmental considerations.

Using case studies and real process data, the Whitepaper highlights the key parameters for improving process efficiency and reducing resource consumption. A systematic comparison of available resins allows for the identification of the most suitable option, ensuring optimal process performance. This selection is guided not only by binding capacity and selectivity, but also by operational parameters such as pressure-flow behaviour, CIP stability, and column lifetime – all of which have a significant impact on productivity and long-term process robustness.