Capillary Electrophoresis

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  • siRNA Silencing of Lamin A and Quantification of the Knockdown Effect via qPCR

    Lamins are structural proteins which form a thin fibrous layer called the nuclear lamina. Currently, three types of lamins are known in mammalian cells: lamin A, B, and C. Lamins may be involved in DNA replication, chromatin organization, differentiation, nuclear structural support, nuclear envelope reassembly, and several other processes. Mutations of lamin A can cause muscular dystrophy, dilated cardiomyopathy, familiar partial lipodystrophy [3], and other disorders. For the detailed analysis of the different functions of this more than 70 kDa large protein, the expression should be blocked. more

  • Half a Century of Chromatography with Agilent and Predecessors

    Twsett originated the chromatography with his work about the separation of chlorophyll in 1903. For some time it was relatively quite around this new technology. Exceptions were the workings of Kuhn and Lederer (1931) as well as of Hesse (1936 and 1942). A break through succeeded to E. Cremer during the 40ies, but hardly considered caused by the war events, what however in the years 1950/51 by the conception of the gas chromatography suddenly changed and involved a rapid development more

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