20 Current white paper about the topic proteins

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Field-Flow Fractionation - The Universal Separation Principle for Particle and Macromolecule Characterization

22-09-2010

High molecular weight polymers, bio molecules or particles in a range of a few nanometers up to several microns are more and more important in science and technology. Many applications are based on the specific properties of these materials. For this reason, an exact knowledge of the molar ...

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Automated Dynamic Light Scattering

09-11-2009

INTRODUCTION From the late 1980s commercial Dynamic Light Scattering (DLS) detectors started being used in commercial biophysical laboratories throughout the world. These instruments enabled size measurements of small volumes of proteins and other biomaterials in solution to be made, with ...

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Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus

Z' value of 0.92 indicates a highly robust assay combined with high quality instrumentation

27-04-2009

Introduction Immunoglobulins or antibodies are proteins that are involved in the immune response. They bind with high affinity at exogenous substances (antigens). Immunoglobulins (Ig) are classified according to the structure. The most present antibody in plasma is IgG. It is part of the ...

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Predictor(TM) hERG Fluorescence Polarization Assay Kit performed on the PHERAstar/PHERAstar Plus

28-08-2008

Simple, fast and cost effective alternative to electrophysiological hERG testing Uses fluorescence polarization rather than a radioligand method Results show great similarity to traditional hERG testing methods Introduction The potential for cardiotoxic side-effects continues to challenge ...

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High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests

08-05-2008

Abstract: For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly ...

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Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

08-05-2008

NanoOrange® Assay for protein quantitation performed on the FLUOstar OPTIMA High and small concentration range evaluated Kit can easily be adapted for high throughput measurements #img4388# Introduction The field of proteomics has expanded dramatically in recent years with research on a ...

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Enzymatic Protein Digest for Mass-spectrometric Analysis

26-09-2007

Introduction The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or ...

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Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

26-09-2007

Introduction Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are ...

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Ultrafast De Novo Sequencing of Corynebacterium urealyticum Using the Genome Sequencer 20 System

22-08-2007

Introduction A microbial genome sequence provides a wealth of data and specific information that cannot be obtained by other experimental approaches. A genome sequence can be regarded as the starting point for detailed bioinformatics analysis, metabolic reconstruction and systematic ...

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Epitope Mapping Using the In Vitro Rapid Translation System RTS 100

21-08-2007

The Rapid Translation System (RTS) represents a cell-free system that allows efficient production of protein from linear DNA sequences. This article gives a brief outline of the use of the RTS technology in linear epitope mapping and shows its promise for reducing time-consuming cloning and ...

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