Microscopy - 3 News by International society for optics and photonics (SPIE)
High-throughput computational microscopy imaging
A new approach for high-throughput quantitative phase microscopy shows potential for delineating subcellular structures in large-scale cell studies
09-Nov-2022 - Cell organelles are involved in a variety of cellular life activities. Their dysfunction is closely related to the development and metastasis of cancer. Exploration of subcellular structures and their abnormal states facilitates insights into the mechanisms of pathologies, which may enable early ...
Overcoming the optical resolution limit
Microsphere assistance enables interferometric topography measurements
03-Nov-2022 - When measuring with light, the lateral extent of the structures that can be resolved by an optical imaging system is fundamentally diffraction limited. Overcoming this limitation is a topic of great interest in recent research, and several approaches have been published in this area. In a recent ...
Frequency-domain STED microscopy for selective background noise suppression
Novel method suppresses background noise in STED microscopy selectively and effectively, with potential for integration into other dual-beam point-scanning techniques
08-Jul-2022 - Nanoscopy describes the ability to see beyond the generally accepted optical limit of 200–300 nm. Stimulated emission depletion (STED) microscopy, developed by Stefan W. Hell and Jan Wichmann in 1994, and experimentally demonstrated by Hell and Thomas Klar in 1999, is a superresolution technique ...
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