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Field-Flow Fractionation - The Universal Separation Principle for Particle and Macromolecule Characterization

22-09-2010

High molecular weight polymers, bio molecules or particles in a range of a few nanometers up to several microns are more and more important in science and technology. Many applications are based on the specific properties of these materials. For this reason, an exact knowledge of the molar mass or particle sizes distribution is essential for later application as well as for optimization of the production processes or quality control. In addition the knowledge about the chain structure of macromolecules or biopolymers as well as about the shape of particles is often necessary. For this reason those samples, which often can be very complex systems, have to be separated according to parameters such as e.g. hydrodynamic volume or molecular weight. After the separation process the resulting narrow dispersed fractions are detected with different techniques....

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New Silicone Technologies for Skin Care

16-11-2009

In today's competitive skin care market, consumers look for a range of benefits, superior aesthetics and cost effectiveness. In response, formulators strive to create products that are multifunctional, easy to use and effective in small quantities. At the same time, the most successful products deliver benefits that stand out from those of the competition. Creams, lotions and color cosmetics must be easy to spread and comfortable to wear, without feeling tacky or greasy. Silicones have been popular ingredients in skin care products for more than fifty years. These versatile materials are known for their emolliency, wetting and spreading characteristics and ability to provide unique aesthetics. As technology continues to evolve, silicones increasingly offer multifunctional, high performance properties. This article reviews some of the newest silicone technologies available for the formulation of innovative skin care products...

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Automated Dynamic Light Scattering

09-11-2009

DLS analysis is a widely applied, non-invasive method for measuring the effective size of molecules in solution. This technique yields valuable information for the characterization of molecules and particles. It facilitates formerly laborious and time consuming measurements, e.g. screening for aggregates or determining optimal crystallization conditions for proteins. The Wyatt Plate Reader is the instrument of choice for any application where molecular size, stability and aggregation are of interest. It reads industry standard microplates with up to 1536 samples automatically and temperature-controlled, if desired. The applications of the technique are wide ranging and include many of the fastest growing areas of research - proteins, biomaterials, liposomes and micelles as well as the established uses for particle sizing and aggregation detection. Conscious of the numerous characterization challenges in the area of drug delivery systems and therapeutic antibodies...

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High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests

08-05-2008

For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic techniques for quantitative monitoring of these biomarkers are required. In this work, the development of a high sensitive assay for follistatin based on the Imperacer® technology is described. The quantification limit in human serum was found at 60 pg/ml in only 6 µl sample volume. With this initial assay we demonstrate the first application in a set of analytical tests for a high sensitive myostatin pathway related profiling....

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Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

08-05-2008

The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three labelled samples can be loaded on a single gel for comparison. Assay methods for determining protein quantitation include absorbance at 280 nm(1), the Bradford assay(2), Lowry assay(3), BCA method(4) and more sensitive assays such as Fluoroprofile® (Sigma-Aldrich) and NanoOrange® (Molecular Probes®)5()....

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Bradford Assay Performed on BMG LABTECH´s FLUOstar Omega with new Evaluation Software

28-02-2008

Determining the protein concentration of samples is a necessary and often used method in biochemistry. Different colorimetric protein assays have been developed. The most commonly used methods are the Bradford assay, the Lowry assay and the BCA assay. In this application note we demonstrate how to determine the protein concentration of samples by using the Bradford assay and the new FLUOstar Omega....

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Sensible and self-monitoring

Online process for soft water control

27-11-2007

For the generation of soft water are mostly used ion exchanging devices with generation of sodium chloride since the beginning of the Sixties. The generated soft water quality and soft water quantity are depending thereby on the static and dynamic characteristics of the softening plant in operation....

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Enzymatic Protein Digest for Mass-spectrometric Analysis

26-09-2007

The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional electrophoresis must be proteolytically cleaved in the course of sample preparation for identification and characterization. A reproducible cleavage pattern of digested proteins is a prerequisite for the unambiguous identification of these proteins by mass spectrometry (MS)....

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High Pure PCR Cleanup Micro Kit - Novel Flexibility for Nucleic Acid Purification

19-09-2007

Manual nucleic acid isolation with High Pure products is long approved by the scientific community. The product portfolio includes kits for isolation of genomic RNA and DNA, plasmid DNA as well as purification of reaction products. Key goal for all products is to offer optimal solutions suited for many different sample materials in order to reduce the number of different nucleic acid isolation kits necessary in modern molecular biology labs....

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Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System

12-09-2007

Alexandre Evrard1,2, Caroline Raynal1,2, Jean-Christophe Boyer2, Lionel Le Gallic2, and Serge Lumbroso1,2 1Institut de Génomique Fonctionnelle, Département d'Oncologie Moléculaire et Cellulaire, Montpellier, France, 2Laboratoire de Biochimie, Hôpital Carémeau, CHU Nîmes, France *Corresponding author...

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