13 Current white paper about the topic expression

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High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests

08-May-2008

For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological mat

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Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology

29-Aug-2007

MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18-25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA tr

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The Universal ProbeLibrary - a Versatile Tool for Quantitative Expression Analysis in the Zebrafish

29-Aug-2007

The teleost zebrafish is a widely used model organism for the study of developmental and disease-related processes in vertebrates, including humans. Understanding these processes at a molecular and cellular level frequently requires the estimation of

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Quantification of Zea mays mRNAs by Real-Time PCR Using the Universal ProbeLibrary

24-Aug-2007

The Universal ProbeLibrary (UPL) is a detection system for real-time PCR based on locked nucleic acid (LNA) probes.

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Epitope Mapping Using the In Vitro Rapid Translation System RTS 100

21-Aug-2007

The Rapid Translation System (RTS) represents a cell-free system that allows efficient production of protein from linear DNA sequences. This article gives a brief outline of the use of the RTS technology in linear epitope mapping and shows its promis

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Superior Transfection of Human Embryonic Stem Cells with FuGENE® HD Transfection Reagent

21-Aug-2007

Gene manipulation techniques are potentially powerful tools in many applications for human embryonic stem (ES) cell research

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Fugene® HD Transfection Reagent: Superior Performance for Challenging Expression Studies

16-Aug-2007

The ability to efficiently introduce foreign molecules into cells constitutes an important tool in any laboratory and facilitates studies of gene regulation and protein function. A plethora of transfection methods have been developed for transfer of

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Optimization of Transfection Conditions for the Human HL-60 Promyelocytic Leukemic T-Cell Line Using FuGENE® HD Transfection Reagent

15-Aug-2007

Transfection is commonly performed to introduce many different types of molecules (plasmid DNA, RNA, siRNA, miRNA) into eukaryotic cell lines. Once inside, these molecules can be expressed in a transient or a stable fashion. In the case of transient

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To Probe or Not to Probe

Comparison of qPCR Assays Using the LightCycler® 2.0 Instrument and either SYBR Green I Intercalation or the Universal ProbeLibrary as Detection Format

10-Aug-2007

In our efforts to measure the expression levels of the thyroid hormone receptor ß2 in small areas of mouse brain, we encountered primer-dimer prob­lems when using SYBR Green I assays. With the Universal ProbeLibrary and the LightCycler® 2.0 Instrumen

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MagNA Pure Compact RNA Isolation Kit: Isolation of High-Quality Total RNA from a Broad Range of Sample Material

08-Aug-2007

The MagNA Pure Compact Instrument together with the new MagNA Pure Compact RNA Isolation Kit can be used for automated isolation of total RNA from blood, blood cells, tissue, and cultured cells. The MagNA Pure Compact System perfectly fits into Roche

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