10 Current white paper about the topic base

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Automated Dynamic Light Scattering

09-11-2009

INTRODUCTION From the late 1980s commercial Dynamic Light Scattering (DLS) detectors started being used in commercial biophysical laboratories throughout the world. These instruments enabled size measurements of small volumes of proteins and other biomaterials in solution to be made, with ...

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Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System

12-09-2007

Introduction The purpose of the study was to screen sequence variations of the cytidine deaminase (CDA) gene in tumor samples. CDA plays a key role in the tumoral and hepatic catabolism and inactivation of gemcitabine, a nucleoside analog extensively used in chemotherapeutic treatment of ...

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Metagenomics Analysis Using the Genome Sequencer FLX System

05-09-2007

The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing. The ...

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The New Genome Sequencer FLX System

More flexibility, more breakthrough applications - Roche continues to unlock the sequencing secrets that will change the world

27-08-2007

Introduction In 2005, the Genome Sequencer 20 System was introduced into the global life science market. Based on the revolutionary 454 sequencing technology, this system is able to generate hundreds of thousands of sequence reads in a few hours at a fraction of costs compared with the ...

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Quantitative Detection of Legionella pneumophila in Water Samples: Assay Transfer to the LightCycler® 480 Real-Time PCR System

22-08-2007

An assay to detect and quantify Legionella spp. in water samples was successfully transferred from a capillary-based LightCycler® Instrument to the novel multiwell plate-based LightCycler® 480 System. A previously described protocol was easily adapted by modifying the fluorescent label of the ...

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Ultrafast De Novo Sequencing of Corynebacterium urealyticum Using the Genome Sequencer 20 System

22-08-2007

Introduction A microbial genome sequence provides a wealth of data and specific information that cannot be obtained by other experimental approaches. A genome sequence can be regarded as the starting point for detailed bioinformatics analysis, metabolic reconstruction and systematic ...

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LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study

09-08-2007

Introduction The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. #img4150# In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, software, and reagents) ...

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cDNA Synthesis with the Transcriptor First Strand cDNA Synthesis Kit from Formalin-Fixed Paraffin-Embedded Tissue (FFPET) Samples

11-06-2007

Partial degradation of RNA is a common problem when working with FFPET samples. Semi-­quantitative RT-PCR assays amplifying the 5´- and 3´-end sequences of the housekeeping gene b-actin present a solution: calculating the ratio of the 3´- to 5´-amplicon allows ­assessment of RNA integrity. An ...

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Making the connection - particle size, size distribution and rheology

23-01-2007

A number of factors influence the rheology of a suspension, including particle size, particle size distribution, and the volume fraction of solids present. Here we examine the relationship between rheology and particle size parameters. Commonly used rheological terms are described and we ...

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The Determination of Hazardous Substances According to RoHS and WEEE Directives

28-08-2005

Abstract1st July 2006 is an important deadline for the electro- and electronics industries. This date bans hazardous substances like lead, cadmium, mercury, hexavalent chromium and polybrominated biphenyls and diphenyl ethers from electro- and electronic equipment. This restriction, even ...

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