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Polyolefins: Determination of Molecular Weight and Branching Structure by GPC/SEC

16-Jun-2008

Polyethylene and Polypropylene are so-called Polyolefins. They are produced in large amounts every year. Depending on the process and catalyst used linear or branched polymers can be produced. In case of e. g. Polyethylene there are at least three po

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Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

08-May-2008

The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three lab

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High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests

08-May-2008

For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological mat

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Nature's Ingenuity Shows the Way

FusionOpticsTM - The New Dimension of Stereomicroscopy

17-Mar-2008

Up to 80 percent of our experience of our visual environment takes place via our visual perception. Without spatial vision, we would hardly be able to stay oriented. In recent decades, the neurosciences have gained many insights into the complex proc

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Bradford Assay Performed on BMG LABTECH´s FLUOstar Omega with new Evaluation Software

28-Feb-2008

Determining the protein concentration of samples is a necessary and often used method in biochemistry. Different colorimetric protein assays have been developed. The most commonly used methods are the Bradford assay, the Lowry assay and the BCA assay

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Sensible and self-monitoring

Online process for soft water control

04-Dec-2007

For the generation of soft water are mostly used ion exchanging devices with generation of sodium chloride since the beginning of the Sixties. The generated soft water quality and soft water quantity are depending thereby on the static and dynamic ch

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Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

26-Sep-2007

Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfect

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Enzymatic Protein Digest for Mass-spectrometric Analysis

26-Sep-2007

The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinit

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Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System

19-Sep-2007

One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleo­tides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same

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Genotypes in Smokers: Correlations with Smoking Behavior

12-Sep-2007

Smoking behavior is influenced by both genetic and environmental factors. Moreover, smoking initiation and smoking persistence have a heritability of at least 50%. A large body of work has been dedicated to associating multiple genetic markers of neu

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