15 Current white paper

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"Green Chemstry" in the Laboratory

Using mills as efficient and ecologically friendly synthesis reactors

25-06-2008

The term "green chemistry" is commonly used to describe chemical procedures which meet specific requirements with regards to the conservation of resources and protection of the environment. The objective is to avoid undesired by-products by selecting adequate reaction procedures and media. A ...

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High Pure PCR Cleanup Micro Kit - Novel Flexibility for Nucleic Acid Purification

19-09-2007

Manual nucleic acid isolation with High Pure products is long approved by the scientific community. The product portfolio includes kits for isolation of genomic RNA and DNA, plasmid DNA as well as purification of reaction products. Key goal for all products is to offer optimal solutions suited ...

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The Universal ProbeLibrary - a Versatile Tool for Quantitative Expression Analysis in the Zebrafish

29-08-2007

The teleost zebrafish is a widely used model organism for the study of developmental and disease-related processes in vertebrates, including humans. Understanding these processes at a molecular and cellular level frequently requires the estimation of gene expression levels. Quantitative real-time ...

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Quantification of Zea mays mRNAs by Real-Time PCR Using the Universal ProbeLibrary

24-08-2007

The Universal ProbeLibrary (UPL) is a detection system for real-time PCR based on locked nucleic acid (LNA) probes. We tested the efficiency of the UPL for the quantification of maize transcripts on two detection platforms. The results indicate that the UPL is an attractive alternative to assays ...

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To Probe or Not to Probe

Comparison of qPCR Assays Using the LightCycler® 2.0 Instrument and either SYBR Green I Intercalation or the Universal ProbeLibrary as Detection Format

10-08-2007

In our efforts to measure the expression levels of the thyroid hormone receptor ß2 in small areas of mouse brain, we encountered primer-dimer prob­lems when using SYBR Green I assays. With the Universal ProbeLibrary and the LightCycler® 2.0 Instrument we were able to solve the primer-dimer ...

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LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study

09-08-2007

The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, software, and reagents) in an academic laboratory ...

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MagNA Pure Compact RNA Isolation Kit: Isolation of High-Quality Total RNA from a Broad Range of Sample Material

08-08-2007

The MagNA Pure Compact Instrument together with the new MagNA Pure Compact RNA Isolation Kit can be used for automated isolation of total RNA from blood, blood cells, tissue, and cultured cells. The MagNA Pure Compact System perfectly fits into Roche Applied Science's integrated solution for ...

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siRNA Silencing of Lamin A and Quantification of the Knockdown Effect via qPCR

08-08-2007

Lamins are structural proteins which form a thin fibrous layer called the nuclear lamina. Currently, three types of lamins are known in mammalian cells: lamin A, B, and C. Lamins may be involved in DNA replication, chromatin organization, differentiation, nuclear structural support, nuclear ...

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Detection of Avian H5N1 Virus with the LightCycler® Instrument

07-08-2007

The avian H5N1 influenza virus has killed thousands of birds and caused several fatal cases in humans in Asia and Turkey. A fast and reliable test to identify the virus is urgently required for an effective epedemiological control, and associated medical research activities. RNA viruses mutate ...

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Rapid Screening by Direct Colony PCR Using the FastStart PCR Master

11-06-2007

The FastStart PCR Master is a convenient and ideal tool for direct colony PCR. Intact bacteria can be analyzed directly without prior template purification, FastStart PCR Master is therefore the tool of choice for rapid screening of cloning experiments. In this paper, we describe the use of the ...

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