13 Current white paperrss
Using mills as efficient and ecologically friendly synthesis reactors
The term "green chemistry" is commonly used to describe chemical procedures which meet specific requirements with regards to the conservation of resources and protection of the environment. The objective is to avoid undesired by-products by selecting adequate reaction procedures and media. A ...
The teleost zebrafish is a widely used model organism for the study of developmental and disease-related processes in vertebrates, including humans. Understanding these processes at a molecular and cellular level frequently requires the estimation of
The Universal ProbeLibrary (UPL) is a detection system for real-time PCR based on locked nucleic acid (LNA) probes. We tested the efficiency of the UPL for the quantification of maize transcripts on two detection platforms. The results indicate that
Comparison of qPCR Assays Using the LightCycler® 2.0 Instrument and either SYBR Green I Intercalation or the Universal ProbeLibrary as Detection Format
In our efforts to measure the expression levels of the thyroid hormone receptor ß2 in small areas of mouse brain, we encountered primer-dimer problems when using SYBR Green I assays. With the Universal ProbeLibrary and the LightCycler® 2.0 Instrument we were able to solve the primer-dimer ...
The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, s
Lamins are structural proteins which form a thin fibrous layer called the nuclear lamina. Currently, three types of lamins are known in mammalian cells: lamin A, B, and C. Lamins may be involved in DNA replication, chromatin organization, differentiation, nuclear structural support, nuclear ...
The MagNA Pure Compact Instrument together with the new MagNA Pure Compact RNA Isolation Kit can be used for automated isolation of total RNA from blood, blood cells, tissue, and cultured cells. The MagNA Pure Compact System perfectly fits into Roche
The avian H5N1 influenza virus has killed thousands of birds and caused several fatal cases in humans in Asia and Turkey. A fast and reliable test to identify the virus is urgently required for an effective epedemiological control, and associated med
The FastStart PCR Master is a convenient and ideal tool for direct colony PCR. Intact bacteria can be analyzed directly without prior template purification, FastStart PCR Master is therefore the tool of choice for rapid screening of cloning experiments. In this paper, we describe the use of the ...
FastStart DNA MasterPLUS SYBR Green I Kit in Highly Sensitive Transcript Quantification
Real-time reverse transcription-PCR (RT-PCR) is considered the method of choice for any experiments requiring sensitive, specific, and reproducible quantification of mRNA molecules of interest. It is an essential tool for basic research, molecular medicine, and biotechnology.