39 Current white paper

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Automated Dynamic Light Scattering

09-11-2009

DLS analysis is a widely applied, non-invasive method for measuring the effective size of molecules in solution. This technique yields valuable information for the characterization of molecules and particles. It facilitates formerly laborious and time consuming measurements, e.g. screening for aggregates or determining optimal crystallization conditions for proteins. The Wyatt Plate Reader is the instrument of choice for any application where molecular size, stability and aggregation are of interest. It reads industry standard microplates with up to 1536 samples automatically and temperature-controlled, if desired. The applications of the technique are wide ranging and include many of the fastest growing areas of research - proteins, biomaterials, liposomes and micelles as well as the established uses for particle sizing and aggregation detection. Conscious of the numerous characterization challenges in the area of drug delivery systems and therapeutic antibodies...

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Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus

Z' value of 0.92 indicates a highly robust assay combined with high quality instrumentation

27-04-2009

Immunoglobulins or antibodies are proteins that are involved in the immune response. That makes them interesting drug targets. This application note shows the detection of the immunoglobulin IgG using an AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus. The AlphaLISA® technology utilizes oxygen to start a cascade of chemical reactions resulting in a luminescence signal at 615 nm. Sensitivity and Z´values obtained in different plate formats are presented....

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Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer

25-08-2008

Nicotinamide Adenine Dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADP+) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) have been known to play vital roles in energy metabolism, antioxidation, and reductive biosynthesis. In recent years, these co-factors have been shown to be involved in other physiological functions as well, including aging, oxidative stress, intracellular calcium homeostasis, ROS production, cell death and gene expression....

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Particle Analysis: Quality Control of Catalysts with Digital Image Processing

24-06-2008

Catalysts are widely used as an important auxiliary material in the chemical and petrochemical industry. They are able to accelerate chemical reactions by lowering the activation energy, i.e. they change reaction kinetics. Thus, using catalysts in industrial processes has the advantage of higher productivity combined with less energy consumption....

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High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests

08-05-2008

For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic techniques for quantitative monitoring of these biomarkers are required. In this work, the development of a high sensitive assay for follistatin based on the Imperacer® technology is described. The quantification limit in human serum was found at 60 pg/ml in only 6 µl sample volume. With this initial assay we demonstrate the first application in a set of analytical tests for a high sensitive myostatin pathway related profiling....

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Nature's Ingenuity Shows the Way

FusionOpticsTM - The New Dimension of Stereomicroscopy

17-03-2008

Up to 80 percent of our experience of our visual environment takes place via our visual perception. Without spatial vision, we would hardly be able to stay oriented. In recent decades, the neurosciences have gained many insights into the complex processes by which our brain's visual cortex and cerebral cortex do the fascinating job of processing the signals originating from the eyes into an image. A study carried out jointly by Leica Microsystems and the Institute of Neuroinformatics at the University of Zurich and Swiss Federal Institute of Technology showed how flexible and powerfull our brain joins visual signals to create an optimal spatial image. The results provided the basis for an innovation in stereomicroscopy which, in terms of resolution and focus depth, has broken through limits that were previously impossible to overcome....

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Bradford Assay Performed on BMG LABTECH´s FLUOstar Omega with new Evaluation Software

28-02-2008

Determining the protein concentration of samples is a necessary and often used method in biochemistry. Different colorimetric protein assays have been developed. The most commonly used methods are the Bradford assay, the Lowry assay and the BCA assay. In this application note we demonstrate how to determine the protein concentration of samples by using the Bradford assay and the new FLUOstar Omega....

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Enzymatic Protein Digest for Mass-spectrometric Analysis

26-09-2007

The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional electrophoresis must be proteolytically cleaved in the course of sample preparation for identification and characterization. A reproducible cleavage pattern of digested proteins is a prerequisite for the unambiguous identification of these proteins by mass spectrometry (MS)....

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Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System

19-09-2007

One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleo­tides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR). Ideally, a real-time multiplex PCR should be able to detect, differentiate, and provide a quantitative result for many different targets without a single target influencing the detection of one of the others (cross-talk) and without loss of sensitivity. It is evident that due to the limited number of fluorophoric labels available and the significant overlap in their emission spectra, quantification of multiplex reaction products is difficult and often not possible for more than two targets....

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Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System

12-09-2007

Alexandre Evrard1,2, Caroline Raynal1,2, Jean-Christophe Boyer2, Lionel Le Gallic2, and Serge Lumbroso1,2 1Institut de Génomique Fonctionnelle, Département d'Oncologie Moléculaire et Cellulaire, Montpellier, France, 2Laboratoire de Biochimie, Hôpital Carémeau, CHU Nîmes, France *Corresponding author...

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