16 Current white paper
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Z' value of 0.92 indicates a highly robust assay combined with high quality instrumentation
27-04-2009
Introduction Immunoglobulins or antibodies are proteins that are involved in the immune response. They bind with high affinity at exogenous substances (antigens). Immunoglobulins (Ig) are classified according to the structure. The most present antibody in plasma is IgG. It is part of the ...
28-08-2008
Simple, fast and cost effective alternative to electrophysiological hERG testing Uses fluorescence polarization rather than a radioligand method Results show great similarity to traditional hERG testing methods Introduction The potential for cardiotoxic side-effects continues to challenge ...
08-05-2008
Abstract: For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly ...
08-05-2008
NanoOrange® Assay for protein quantitation performed on the FLUOstar OPTIMA High and small concentration range evaluated Kit can easily be adapted for high throughput measurements #img4388# Introduction The field of proteomics has expanded dramatically in recent years with research on a ...
26-09-2007
Introduction Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are ...
19-09-2007
Introduction One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleotides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR) ...
12-09-2007
Introduction The purpose of the study was to screen sequence variations of the cytidine deaminase (CDA) gene in tumor samples. CDA plays a key role in the tumoral and hepatic catabolism and inactivation of gemcitabine, a nucleoside analog extensively used in chemotherapeutic treatment of ...
29-08-2007
MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18–25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA transcripts annotated. Although miRNAs ...
23-08-2007
The LightCycler® Real-time PCR Systems provide fast, accurate, and versatile platforms for genetic variation research. They feature the temperature homogeneity and the optical characteristics re-quired for high-performance melting curve analysis. The plate-based LightCycler® 480 System now ...
22-08-2007
An assay to detect and quantify Legionella spp. in water samples was successfully transferred from a capillary-based LightCycler® Instrument to the novel multiwell plate-based LightCycler® 480 System. A previously described protocol was easily adapted by modifying the fluorescent label of the ...
