16 Current white paperrss
Z' value of 0.92 indicates a highly robust assay combined with high quality instrumentation
Immunoglobulins or antibodies are proteins that are involved in the immune response. That makes them interesting drug targets. This application note shows the detection of the immunoglobulin IgG using an AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus. The AlphaLISA® technology ...
The potential for cardiotoxic side-effects continues to challenge the development of small molecule based therapies. These intolerable side-effects are often precipitated by drug-induced long QT syndrome (LQT), which is often linked to blocking the human ether-a-go-go related gene (hERG) ...
For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic ...
The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three lab
Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few ...
One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleotides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR). Ideally, a ...
Alexandre Evrard1,2, Caroline Raynal1,2, Jean-Christophe Boyer2, Lionel Le Gallic2, and Serge Lumbroso1,2 1Institut de Génomique Fonctionnelle, Département d'Oncologie Moléculaire et Cellulaire, Montpellier, France, 2Laboratoire de Biochimie, Hôpita
MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18-25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA tr
The LightCycler® Real-time PCR Systems provide fast, accurate, and versatile platforms for genetic variation research. They feature the temperature homogeneity and the optical characteristics re-quired for high-performance melting curve analysis. The plate-based LightCycler® 480 System now ...
An assay to detect and quantify Legionella spp. in water samples was successfully transferred from a capillary-based LightCycler® Instrument to the novel multiwell plate-based LightCycler® 480 System. A previously described protocol was easily adapte