26-09-2007
Introduction The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or ...
19-09-2007
Introduction One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleotides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR) ...
12-09-2007
Introduction The purpose of the study was to screen sequence variations of the cytidine deaminase (CDA) gene in tumor samples. CDA plays a key role in the tumoral and hepatic catabolism and inactivation of gemcitabine, a nucleoside analog extensively used in chemotherapeutic treatment of ...
05-09-2007
The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing. The ...
28-08-2007
High-resolution melting (HRM) is a novel, homogeneous, post-PCR method, enabling genomic researchers to analyze genetic variations (SNPs, mutations, methylations) in PCR amplicons. The most important high-resolution melting application is gene scanning - the search for the presence of unknown ...
More flexibility, more breakthrough applications - Roche continues to unlock the sequencing secrets that will change the world
27-08-2007
Introduction In 2005, the Genome Sequencer 20 System was introduced into the global life science market. Based on the revolutionary 454 sequencing technology, this system is able to generate hundreds of thousands of sequence reads in a few hours at a fraction of costs compared with the ...
27-08-2007
A major problem in some areas of cancer research is the difficulty of transfecting a significant number of the cells of interest, while minimizing the cytotoxic or other off-target effects of the transfection event itself. It is well-known that the use of some transfection reagents results in ...
08-08-2007
Introduction The MagNA Pure Compact Instrument together with the new MagNA Pure Compact RNA Isolation Kit can be used for automated isolation of total RNA from blood, blood cells, tissue, and cultured cells. The MagNA Pure Compact System perfectly fits into Roche Applied Science's integrated ...
11-06-2007
Introduction The FastStart PCR Master is a convenient and ideal tool for direct colony PCR. Intact bacteria can be analyzed directly without prior template purification, FastStart PCR Master is therefore the tool of choice for rapid screening of cloning experiments. In this paper, we describe ...
11-06-2007
Partial degradation of RNA is a common problem when working with FFPET samples. Semi-quantitative RT-PCR assays amplifying the 5´- and 3´-end sequences of the housekeeping gene b-actin present a solution: calculating the ratio of the 3´- to 5´-amplicon allows assessment of RNA integrity. An ...
