8 Current white paper

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Field-Flow Fractionation - The Universal Separation Principle for Particle and Macromolecule Characterization

22-09-2010

High molecular weight polymers, bio molecules or particles in a range of a few nanometers up to several microns are more and more important in science and technology. Many applications are based on the specific properties of these materials. For this reason, an exact knowledge of the molar mass ...

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New Silicone Technologies for Skin Care

16-11-2009

In today's competitive skin care market, consumers look for a range of benefits, superior aesthetics and cost effectiveness. In response, formulators strive to create products that are multifunctional, easy to use and effective in small quantities. At the same time, the most successful products ...

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Polyether-Modified Silicones for Cosmetic Applications

20-07-2009

Polyether-modified silicones have a long history of use in the personal care industry as emulsifiers and dispersants, as well as stabilizers for water-in-oil systems. However, because of their external phase type, water-in-oil systems tend to display poor aesthetics, such as greasiness, tackiness ...

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Enzymatic Protein Digest for Mass-spectrometric Analysis

26-09-2007

The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional ...

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Real-Time PCR Quality Control for Gene Expression

Profiling Using the LightCycler® 480 System

28-08-2007

Quantitative real-time PCR (qPCR) has become the de facto standard for nucleic acid quantification. This achievement is due in large part to its sensitivity, specificity, accuracy, large dynamic range of linear quantification, and its speed. The qPCR technology has matured to a ready-to-use ...

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Analysis of the DNA Methylation Patterns at the BRCA1 CpG Island

20-08-2007

Germ-line alterations of the BRCA1 gene confer a lifetime risk of 40% for ovarian cancers and of 40%-80% for ­breast cancers. It is likely that BRCA1 acts as a tumor suppressor gene. BRCA1 involvement in breast cancers does not seem to be restricted to familial cancers. Despite the absence of ...

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Quantification of BRCA1 Expression Level Using Standard RT-PCR Reagents - a Sensitive Method for the Detection of Low Amounts of Transcripts

17-08-2007

Mutations in the breast cancer susceptibility gene, BRCA1, occur in the majority of families with multiple members affected with breast or ovarian cancer. Women who inherit a BRCA1 mutation have 40%-80% risk of developing breast cancer or ovarian cancer. However, up to 90% of breast cancers are ...

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Fugene® HD Transfection Reagent: Superior Performance for Challenging Expression Studies

16-08-2007

The ability to efficiently introduce foreign molecules into cells constitutes an important tool in any laboratory and facilitates studies of gene regulation and protein function. A plethora of transfection methods have been developed for transfer of DNA into mammalian and animal cells. Those ...

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