10 Current white paper

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Automated Dynamic Light Scattering

09-11-2009

DLS analysis is a widely applied, non-invasive method for measuring the effective size of molecules in solution. This technique yields valuable information for the characterization of molecules and particles. It facilitates formerly laborious and time consuming measurements, e.g. screening for aggregates or determining optimal crystallization conditions for proteins. The Wyatt Plate Reader is the instrument of choice for any application where molecular size, stability and aggregation are of interest. It reads industry standard microplates with up to 1536 samples automatically and temperature-controlled, if desired. The applications of the technique are wide ranging and include many of the fastest growing areas of research - proteins, biomaterials, liposomes and micelles as well as the established uses for particle sizing and aggregation detection. Conscious of the numerous characterization challenges in the area of drug delivery systems and therapeutic antibodies...

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Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler® 480 System

12-09-2007

Alexandre Evrard1,2, Caroline Raynal1,2, Jean-Christophe Boyer2, Lionel Le Gallic2, and Serge Lumbroso1,2 1Institut de Génomique Fonctionnelle, Département d'Oncologie Moléculaire et Cellulaire, Montpellier, France, 2Laboratoire de Biochimie, Hôpital Carémeau, CHU Nîmes, France *Corresponding author...

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Metagenomics Analysis Using the Genome Sequencer FLX System

05-09-2007

The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing. The Genome Sequencer FLX is built upon 454 Sequencing technology that enables long read lengths and very high single-read accuracy. One application in which the technology is accelerating the field's understanding is metagenomics....

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The New Genome Sequencer FLX System

More flexibility, more breakthrough applications - Roche continues to unlock the sequencing secrets that will change the world

27-08-2007

In 2005, the Genome Sequencer 20 System was introduced into the global life science market. Based on the revolutionary 454 sequencing technology, this system is able to generate hundreds of thousands of sequence reads in a few hours at a fraction of costs compared with the traditional Sanger technology....

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Quantitative Detection of Legionella pneumophila in Water Samples: Assay Transfer to the LightCycler® 480 Real-Time PCR System

22-08-2007

An assay to detect and quantify Legionella spp. in water samples was successfully transferred from a capillary-based LightCycler® Instrument to the novel multiwell plate-based LightCycler® 480 System. A previously described protocol was easily adapted by modifying the fluorescent label of the detection probe to match the filter set of the LightCycler® 480 Instrument. While reproducibility and analytical sensitivity of the assay were found to be comparable on both systems, the novel plate-based instrument allowed for higher sample throughput and reduced assay time per sample....

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Ultrafast De Novo Sequencing of Corynebacterium urealyticum Using the Genome Sequencer 20 System

22-08-2007

A microbial genome sequence provides a wealth of data and specific information that cannot be obtained by other experimental approaches. A genome sequence can be regarded as the starting point for detailed bioinformatics analysis, metabolic reconstruction and systematic functional examination of all identified genes. In particular, genome sequencing has revealed important information on genes and deduced proteins of human pathogens, including putative virulence factors and potential new drug targets....

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LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study

09-08-2007

The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, software, and reagents) in an academic laboratory setting we scheduled a high-throughput project including gene expression (39 targets in 88 samples = 3,400 expression values) as well as genotyping applications (3,808 genotypes; 14 SNPs in 288 samples). The project, involving five technicians and two graduate students, was accomplished within 15 working days....

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cDNA Synthesis with the Transcriptor First Strand cDNA Synthesis Kit from Formalin-Fixed Paraffin-Embedded Tissue (FFPET) Samples

11-06-2007

Partial degradation of RNA is a common problem when working with FFPET samples. Semi-­quantitative RT-PCR assays amplifying the 5´- and 3´-end sequences of the housekeeping gene b-actin present a solution: calculating the ratio of the 3´- to 5´-amplicon allows ­assessment of RNA integrity. An important step for reproducible quantification is the reverse ­transcription reaction. The ­applicability of the new Transcriptor First Strand cDNA Synthesis Kit to partially degraded FFPET RNA was evaluated. The new kit is compared with the widely used First Strand cDNA Synthesis Kit for RT-PCR (AMV). Under modified reaction ­conditions (temperature, time) the kit reversely transcribes the FFPET samples with high ­efficiency....

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Making the connection - particle size, size distribution and rheology

23-01-2007

A number of factors influence the rheology of a suspension, including particle size, particle size distribution, and the volume fraction of solids present. Here we examine the relationship between rheology and particle size parameters. Commonly used rheological terms are described and we present data from example systems to illustrate key points...

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The Determination of Hazardous Substances According to RoHS and WEEE Directives

28-08-2005

1st July 2006 is an important deadline for the electro- and electronics industries. This date bans hazardous substances like lead, cadmium, mercury, hexavalent chromium and polybrominated biphenyls and diphenyl ethers from electro- and electronic equipment. This restriction, even though in discussion since 1991, has become more and more important from year to year because of tons of electronic waste releasing toxic substances which are accumulated in the environment, the food chain and all human beings. In order to enforce the substances ban and the limitation or substitution of hazardous components, elemental analysis is obviously the most important control measure for monitoring limiting values. This requires precise analytical systems such as X-ray fluorescence, ICP- and atomic absorption spectrometers. These instruments are able to detect trace concentrations of hazardous compounds - for example cadmium, using an atomic absorption spectrometer in the flame atomization mode up to 0.1 mg/L, or using the di...

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