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Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer


microplates (Cat #3635 and #3675). NADP and NADPH from Sigma-Aldrich, US NAD and NADH from Roth, Germany Tris-HCl buffer (pH=7.4) #img4501#A final volume of 300 µl was used for 96-well plates and 100 µl...


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Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader


), the Bradford assay(2), Lowry assay(3), BCA method(4) and more sensitive assays such as Fluoroprofile® (Sigma-Aldrich) and NanoOrange® (Molecular Probes®)5(). The measurement of solution absorbance at 280 nm...


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Bradford Assay Performed on BMG LABTECH´s FLUOstar Omega with new Evaluation Software


microplates from Greiner, Frickenhausen, Germany FLUOstar Omega, BMG LABTECH, Offenburg, Germany Bovine Serum Albumin (BSA, Cat. No. A-9647) from Sigma-Aldrich, Taufkirchen, Germany Bradford Reagent (Cat. No. B...


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