Transfection

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White papers Transfection

  • Superiority of FuGENE® HD Transfection Reagent in Minimizing Non-Specific Cellular Interferon Responses

    Many viruses, including human cytomegalovirus (HCMV), encode for genes that can modulate or interfere with the ability of cells to mount an effective antiviral response to infection. Transfection of viral genes into cultures of mammalian cells is an important research tool to evaluate the function of viral gene products. We utilized this approach to identify viral genes involved in modulation of interferon (IFN)-mediated cell signaling pathways. more

  • Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

    Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

  • Enabling Cancer Research with FuGENE® HD Transfection Reagent

    A major problem in some areas of cancer research is the difficulty of transfecting a significant number of the cells of interest, while minimizing the cytotoxic or other off-target effects of the transfection event itself. It is well-known that the use of some transfection reagents results in high levels of expression of the gene of interest in surviving cells, but also in high levels of cell death or other cellular alterations. FuGENE® HD Transfection Reagent overcomes these problems, results in high ­levels of transfection in many cancer cell lines, and thus enables researchers to conduct experiments that have previously not been possible. more

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Websites Transfection

Cell Projects: Electroporation, PCR, HTS, Isohelix DNA Products

Highly innovative Molecular Biology Solutions for Electroporation, PCR, DNA Buccal Swabs, DNA Isolation Kits and other Life Science Technologies. The Cell Projects ™ product division supplies quality plastic and chemical enabling kits for: Electroporation Transfection PCR HTS Isohelix DNA Isolation more

amsbio (AMS Biotechnology) - Your source for life science products and services. Heparanase, Zymolya

AMS Biotechnology (Europe) Ltd Your source for life science products and services. We have over 298,000 products in our catalogue. ISO 9001:2008 reg. Please Select.. By Keyword By Product Code Find an Antibody Find an Assay Kit Find a DNA Find a Protein Find a RNA By Accession Number By Clone Number more

ibidi - cells in focus

FREE SAMPLE PROGRAM Choose up to 3 different Slides for free!    >> more        MEET US AT: July 29, 2010 | Boston, MA LSE Show July 30, 2010 | Cambridge, MA LSE Show Aug 24-26 | Troy, USA ECIS User Meeting >> more IBIDI NEWS LifeAct-Plasmids Novel probe for visualization of Actin cytoskeleton >> mo more

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