Transfection

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White papers Transfection

  • Fugene® HD Transfection Reagent: Superior Performance for Challenging Expression Studies

    The ability to efficiently introduce foreign molecules into cells constitutes an important tool in any laboratory and facilitates studies of gene regulation and protein function. A plethora of transfection methods have been developed for transfer of DNA into mammalian and animal cells. Those methods either utilize the physicochemical properties of the vector-carrier of the DNA or use mammalian viruses. In the first group of methods, some classic techniques are well-known, such as: calcium phosphate, DEAE-dextran, electroporation liposome-mediated delivery, direct microinjection/biolistic particle delivery, and non-liposomal multi-component reagents. Viral vector systems use both DNA (Adeno-) and RNA (Retro-, Lenti-) viruses. more

  • Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

    Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

  • Optimization of Transfection Conditions for the Human HL-60 Promyelocytic Leukemic T-Cell Line Using FuGENE® HD Transfection Reagent

    Transfection is commonly performed to introduce many different types of molecules (plasmid DNA, RNA, siRNA, miRNA) into eukaryotic cell lines. Once inside, these molecules can be expressed in a transient or a stable fashion. In the case of transient transfection, molecules do not integrate into the host cell genome and are only expressed for approximately 24 hours - 96 hours post-transfection. more

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Websites Transfection

Cell Projects: Electroporation, PCR, HTS, Isohelix DNA Products

Highly innovative Molecular Biology Solutions for Electroporation, PCR, DNA Buccal Swabs, DNA Isolation Kits and other Life Science Technologies. The Cell Projects ™ product division supplies quality plastic and chemical enabling kits for: Electroporation Transfection PCR HTS Isohelix DNA Isolation more

amsbio (AMS Biotechnology) - Your source for life science products and services. Heparanase, Zymolya

AMS Biotechnology (Europe) Ltd Your source for life science products and services. We have over 298,000 products in our catalogue. ISO 9001:2008 reg. Please Select.. By Keyword By Product Code Find an Antibody Find an Assay Kit Find a DNA Find a Protein Find a RNA By Accession Number By Clone Number more

ibidi - cells in focus

FREE SAMPLE PROGRAM Choose up to 3 different Slides for free!    >> more        MEET US AT: July 29, 2010 | Boston, MA LSE Show July 30, 2010 | Cambridge, MA LSE Show Aug 24-26 | Troy, USA ECIS User Meeting >> more IBIDI NEWS LifeAct-Plasmids Novel probe for visualization of Actin cytoskeleton >> mo more

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