Transfection

News Transfection

All news on transfection

White papers Transfection

  • Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

    Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

  • Efficient Transfection of Neuroblastoma Cell Lines Using FuGENE® HD Transfection Reagent

    Efficient transfection of cells is a prerequisite for genetic manipulation. However, many cell lines remain hard to transfect with established protocols, hampering consecutive analysis. Here, we compare three different methods (FuGENE® HD Transfection Reagent, transfection reagent L, and calcium phosphate) for the transfection of neuroblastoma cell lines. We show that FuGENE® HD Transfection Reagent is able to transfect all of these cell lines with high efficiency for further analysis. more

  • siRNA Silencing of Lamin A and Quantification of the Knockdown Effect via qPCR

    Lamins are structural proteins which form a thin fibrous layer called the nuclear lamina. Currently, three types of lamins are known in mammalian cells: lamin A, B, and C. Lamins may be involved in DNA replication, chromatin organization, differentiation, nuclear structural support, nuclear envelope reassembly, and several other processes. Mutations of lamin A can cause muscular dystrophy, dilated cardiomyopathy, familiar partial lipodystrophy [3], and other disorders. For the detailed analysis of the different functions of this more than 70 kDa large protein, the expression should be blocked. more

All white papers on transfection

Websites Transfection

Cell Projects: Electroporation, PCR, HTS, Isohelix DNA Products

Highly innovative Molecular Biology Solutions for Electroporation, PCR, DNA Buccal Swabs, DNA Isolation Kits and other Life Science Technologies. The Cell Projects ™ product division supplies quality plastic and chemical enabling kits for: Electroporation Transfection PCR HTS Isohelix DNA Isolation more

amsbio (AMS Biotechnology) - Your source for life science products and services. Heparanase, Zymolya

AMS Biotechnology (Europe) Ltd Your source for life science products and services. We have over 298,000 products in our catalogue. ISO 9001:2008 reg. Please Select.. By Keyword By Product Code Find an Antibody Find an Assay Kit Find a DNA Find a Protein Find a RNA By Accession Number By Clone Number more

ibidi - cells in focus

FREE SAMPLE PROGRAM Choose up to 3 different Slides for free!    >> more        MEET US AT: July 29, 2010 | Boston, MA LSE Show July 30, 2010 | Cambridge, MA LSE Show Aug 24-26 | Troy, USA ECIS User Meeting >> more IBIDI NEWS LifeAct-Plasmids Novel probe for visualization of Actin cytoskeleton >> mo more

All websites on transfection
Your browser is not current. Microsoft Internet Explorer 6.0 does not support some functions on Chemie.DE