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  • Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

    Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

  • Enabling Cancer Research with FuGENE® HD Transfection Reagent

    A major problem in some areas of cancer research is the difficulty of transfecting a significant number of the cells of interest, while minimizing the cytotoxic or other off-target effects of the transfection event itself. It is well-known that the use of some transfection reagents results in high levels of expression of the gene of interest in surviving cells, but also in high levels of cell death or other cellular alterations. FuGENE® HD Transfection Reagent overcomes these problems, results in high ­levels of transfection in many cancer cell lines, and thus enables researchers to conduct experiments that have previously not been possible. more

  • Efficient Transfection of Neuroblastoma Cell Lines Using FuGENE® HD Transfection Reagent

    Efficient transfection of cells is a prerequisite for genetic manipulation. However, many cell lines remain hard to transfect with established protocols, hampering consecutive analysis. Here, we compare three different methods (FuGENE® HD Transfection Reagent, transfection reagent L, and calcium phosphate) for the transfection of neuroblastoma cell lines. We show that FuGENE® HD Transfection Reagent is able to transfect all of these cell lines with high efficiency for further analysis. more

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Cell Projects: Electroporation, PCR, HTS, Isohelix DNA Products

Highly innovative Molecular Biology Solutions for Electroporation, PCR, DNA Buccal Swabs, DNA Isolation Kits and other Life Science Technologies. The Cell Projects ™ product division supplies quality plastic and chemical enabling kits for: Electroporation Transfection PCR HTS Isohelix DNA Isolation more

amsbio (AMS Biotechnology) - Your source for life science products and services. Heparanase, Zymolya

AMS Biotechnology (Europe) Ltd Your source for life science products and services. We have over 298,000 products in our catalogue. ISO 9001:2008 reg. Please Select.. By Keyword By Product Code Find an Antibody Find an Assay Kit Find a DNA Find a Protein Find a RNA By Accession Number By Clone Number more

ibidi - cells in focus

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