Topic toxicity

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• Substance evaluation starts under REACH: The first list of substances published

  The first Community Rolling Action plan (CoRAP) addresses 90 substances that are suspected of posing risk to human health or the environment. The CoRAP list has been prepared in close cooperation with the Member States, taking into account the agreed risk based criteria for the selection of substanc more

• How much caffeine is in your coffee?

  Substantial differences exist in the caffeine and chlorogenic acid contents of espresso coffees that can result in over-consumption by consumers at risk of caffeine toxicity, say scientists in the UK. The team carried out high performance liquid chromatography analysis of 20 commercial espresso coff more

• Engineered antibodies directed against a promising therapeutic target on ovarian cancer cells

  Stealthy and stubborn, ovarian cancer is a particularly vexing malignancy, difficult to diagnose in early stages and difficult to treat once it progresses further. However, research at Fox Chase Cancer Center is now focusing on one of the most promising new approaches to dealing with the disease––us more

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• Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent
  

  Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

• Efficient Transfection of Neuroblastoma Cell Lines Using FuGENE® HD Transfection Reagent
  

  Efficient transfection of cells is a prerequisite for genetic manipulation. However, many cell lines remain hard to transfect with established protocols, hampering consecutive analysis. Here, we compare three different methods (FuGENE® HD Transfection Reagent, transfection reagent L, and calcium phosphate) for the transfection of neuroblastoma cell lines. We show that FuGENE® HD Transfection Reagent is able to transfect all of these cell lines with high efficiency for further analysis. more

• Superior Transfection of Human Embryonic Stem Cells with FuGENE® HD Transfection Reagent
  

  Gene manipulation techniques are potentially powerful tools in many applications for human embryonic stem (ES) cell research. Controlling expression levels of specific genes may improve the efficiency of differentiation induction. Cell types of interest may be collected using marker genes and antibiotic resistance genes. Tracking of ES cells derived after engraftment could be facilitated by tracer genes. It may also be possible to selectively destroy transplanted cells. more

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