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• A non-invasive intracellular 'thermometer' with fluorescent proteins has been created
  

  A team from the Institute of Photonic Sciences (ICFO) has developed a technique to measure internal cell temperatures without altering their metabolism. This finding could be useful when distinguishing healthy cells from cancerous ones, as well as learning more about cellular processes.Temperature c more

• Quartz crystal microbalances enable new microscale analytic technique
  

  A new chemical analysis technique developed by a research group at the National Institute of Standards and Technology (NIST) uses the shifting ultrasonic pitch of a small quartz crystal to test the purity of only a few micrograms of material. Since it works with samples close to a thousand times sma more

• Surfaces and Interfaces: Innovative techniques allow researchers to study interfaces with great precision

  Equipment built by German scientists can be used to study processes at interfaces with great accuracy. In an article published recently in ChemPhysChem, Hans Joachim Freund and co-workers of the Fritz-Haber-Institut in Berlin describe the advancement of four experimental techniques developed in thei more

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• Field-Flow Fractionation - The Universal Separation Principle for Particle and Macromolecule Characterization
  

  High molecular weight polymers, bio molecules or particles in a range of a few nanometers up to several microns are more and more important in science and technology. Many applications are based on the specific properties of these materials. For this reason, an exact knowledge of the molar mass or particle sizes distribution is essential for later application as well as for optimization of the production processes or quality control. In addition the knowledge about the chain structure of macromolecules or biopolymers as well as about the shape of particles is often necessary. For this reason those samples, which often can be very complex systems, have to be separated according to parameters such as e.g. hydrodynamic volume or molecular weight. After the separation process the resulting narrow dispersed fractions are detected with different techniques. more

• Automated Dynamic Light Scattering
  

  DLS analysis is a widely applied, non-invasive method for measuring the effective size of molecules in solution. This technique yields valuable information for the characterization of molecules and particles. It facilitates formerly laborious and time consuming measurements, e.g. screening for aggregates or determining optimal crystallization conditions for proteins. The Wyatt Plate Reader is the instrument of choice for any application where molecular size, stability and aggregation are of interest. It reads industry standard microplates with up to 1536 samples automatically and temperature-controlled, if desired. The applications of the technique are wide ranging and include many of the fastest growing areas of research - proteins, biomaterials, liposomes and micelles as well as the established uses for particle sizing and aggregation detection. Conscious of the numerous characterization challenges in the area of drug delivery systems and therapeutic antibodies more

• Homogeneous IgG AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus
  

  Immunoglobulins or antibodies are proteins that are involved in the immune response. That makes them interesting drug targets. This application note shows the detection of the immunoglobulin IgG using an AlphaLISA® assay performed on BMG LABTECH's PHERAstar Plus. The AlphaLISA® technology utilizes oxygen to start a cascade of chemical reactions resulting in a luminescence signal at 615 nm. Sensitivity and Z´values obtained in different plate formats are presented. more

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