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  • Evotec receives first milestone in Roche biomarker collaboration

    Evotec AG announced the successful achievement of a milestone in its biomarker alliance with Roche. The milestone was achieved on the decision by Roche to use a response prediction marker, identified using Evotec's Proteome Profiling platform, in an extended phase I oncology trial. This is the first more

  • Roche and Molecular Partners enter into alliance

    Roche and Molecular Partners AG announced that they have entered  into a research collaboration and licensing agreement to discover, develop and commercialize several proprietary therapeutics incorporating Molecular Partners’ DARPin® biologics conjugated to toxic agents developed at Roche for the tr more

  • SynenTec To Take Over Roche Cellavista System

    Roche and SynenTec announced that Roche will transfer its Cellavista business to SynenTec, the German company which is the original inventor of the Cellavista System. This agreement was made as a result of the Roche Applied Science decision to focus its business, and guarantees future optimal suppor more

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  • Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System

    One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleo­tides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR). Ideally, a real-time multiplex PCR should be able to detect, differentiate, and provide a quantitative result for many different targets without a single target influencing the detection of one of the others (cross-talk) and without loss of sensitivity. It is evident that due to the limited number of fluorophoric labels available and the significant overlap in their emission spectra, quantification of multiplex reaction products is difficult and often not possible for more than two targets. more

  • Enzymatic Protein Digest for Mass-spectrometric Analysis

    The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional electrophoresis must be proteolytically cleaved in the course of sample preparation for identification and characterization. A reproducible cleavage pattern of digested proteins is a prerequisite for the unambiguous identification of these proteins by mass spectrometry (MS). more

  • Enabling Cancer Research with FuGENE® HD Transfection Reagent

    A major problem in some areas of cancer research is the difficulty of transfecting a significant number of the cells of interest, while minimizing the cytotoxic or other off-target effects of the transfection event itself. It is well-known that the use of some transfection reagents results in high levels of expression of the gene of interest in surviving cells, but also in high levels of cell death or other cellular alterations. FuGENE® HD Transfection Reagent overcomes these problems, results in high ­levels of transfection in many cancer cell lines, and thus enables researchers to conduct experiments that have previously not been possible. more

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