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  • Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

    The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three labelled samples can be loaded on a single gel for comparison. Assay methods for determining protein quantitation include absorbance at 280 nm(1), the Bradford assay(2), Lowry assay(3), BCA method(4) and more sensitive assays such as Fluoroprofile® (Sigma-Aldrich) and NanoOrange® (Molecular Probes®)5(). more

  • Enzymatic Protein Digest for Mass-spectrometric Analysis

    The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional electrophoresis must be proteolytically cleaved in the course of sample preparation for identification and characterization. A reproducible cleavage pattern of digested proteins is a prerequisite for the unambiguous identification of these proteins by mass spectrometry (MS). more

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imaGenes - imaGenes

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Ludesi - provider of software and services for 2D gel image analysis

Search:  Home Software Services Resources Pricing References Corporate FREE Tools REDFIN 2D gel analysis software Improve data robustness, save time, reduce costs, and have more fun when analyzing your 2D gel images! Use our analysis service Request a quality-controlled analysis service directly in more

MagnaMedics Diagnostics B.V.

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