Topic proteomics

News proteomics

• analytica presents biotechnology highlights

  From molecular-biology techniques to forensic DNA analysis: from April 17 – 20 analytica features in-depth information about the latest methods and techniques in laboratory technology, analysis and biotechnology. One of the five halls, hall A3, is devoted entirely to the biotech industry. The topic more

• Agilent Technologies Awards Two Integrated-Biology Research Grants

  Agilent Technologies Inc. announced the winners of the 2011 eMerging Insights grant program: Dr. Michael J. MacCoss of the University of Washington and Dr. Peter J. Park of Harvard University. Each will be awarded $75,000 toward their ongoing research on open source data-integration tools.The first more

• Thermo Fisher Scientific Scientist Dr. Alexander Makarov Receives Inaugural HUPO Science and Technology Award

  Thermo Fisher Scientific Inc. announced that Dr. Alexander Makarov, its director of global research for life sciences mass spectrometry, has received the first HUPO Science and Technology Award from the Human Proteome Organization (HUPO). The award, which honors Dr. Makarov’s principal role in devel more

White papers proteomics

• Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader
  

  The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three labelled samples can be loaded on a single gel for comparison. Assay methods for determining protein quantitation include absorbance at 280 nm(1), the Bradford assay(2), Lowry assay(3), BCA method(4) and more sensitive assays such as Fluoroprofile® (Sigma-Aldrich) and NanoOrange® (Molecular Probes®)5(). more

• Enzymatic Protein Digest for Mass-spectrometric Analysis
  

  The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional electrophoresis must be proteolytically cleaved in the course of sample preparation for identification and characterization. A reproducible cleavage pattern of digested proteins is a prerequisite for the unambiguous identification of these proteins by mass spectrometry (MS). more

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