Proteins

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How can you purify proteins faster than with FPLC?

Proteins can be analyzed or purified up to 3x faster with pressure-resistant glass columns of KNAUER Bioline and specially reinforced agarose media more

News Proteins

  • "Funnel" attracts bonding partners to biomolecule

    Water is a ubiquitous solvent in all life sciences – sometimes referred to as the "matrix of life". Contrary to earlier assumptions, it is not a passive witness of biochemical processes; rather, it participates in them actively. By influencing the movement of water molecules surrounding their bindin more

  • Nanocosmos of cells under the magnifying glass

    Scientists from the University of Würzburg have managed to take a unique look at the membranes of human cells using a new technique. This technique that they have devised makes individual saccharified proteins and lipids visible at the molecular level. Picture a satellite orbiting the Earth and capt more

  • The Interior of a Cell from a Moving Protein’s Point of View

    Numerous obstacles posed by cellular structures hinder protein movements within the cell. Researchers from Heidelberg University and the German Cancer Research Center have succeeded in mapping the intracellular topology by observing proteins in living cells on multiple time and length scales. By dev more

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White papers Proteins

  • siRNA Silencing of Lamin A and Quantification of the Knockdown Effect via qPCR

    Lamins are structural proteins which form a thin fibrous layer called the nuclear lamina. Currently, three types of lamins are known in mammalian cells: lamin A, B, and C. Lamins may be involved in DNA replication, chromatin organization, differentiation, nuclear structural support, nuclear envelope reassembly, and several other processes. Mutations of lamin A can cause muscular dystrophy, dilated cardiomyopathy, familiar partial lipodystrophy [3], and other disorders. For the detailed analysis of the different functions of this more than 70 kDa large protein, the expression should be blocked. more

  • Epitope Mapping Using the In Vitro Rapid Translation System RTS 100

    The Rapid Translation System (RTS) represents a cell-free system that allows efficient production of protein from linear DNA sequences. This article gives a brief outline of the use of the RTS technology in linear epitope mapping and shows its promise for reducing time-consuming cloning and cell-lysis steps. more

  • Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

    The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three labelled samples can be loaded on a single gel for comparison. Assay methods for determining protein quantitation include absorbance at 280 nm(1), the Bradford assay(2), Lowry assay(3), BCA method(4) and more sensitive assays such as Fluoroprofile® (Sigma-Aldrich) and NanoOrange® (Molecular Probes®)5(). more

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GENEART - Your partner from gene synthesis to protein production!

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