Proteins

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How can you purify proteins faster than with FPLC?

Proteins can be analyzed or purified up to 3x faster with pressure-resistant glass columns of KNAUER Bioline and specially reinforced agarose media more

News Proteins

  • Nanoscale Velcro used for Molecule Transport

    Biological membranes are like a guarded border. They separate the cell from the environment and at the same time control the import and export of molecules. The nuclear membrane can be crossed via many tiny pores. Scientists at the Biozentrum and the Swiss Nanoscience Institute at the University of more

  • High-speed snapshots of biomolecules

    Using DESY's synchrotron light source PETRA III, scientists have pioneered a new way to analyse delicate biomolecules. The novel approach, borrowed from a new class of high-intensity X-ray sources called free-electron lasers (FELs), could reveal the atomic structure of proteins that were previously more

  • New atom-scale knowledge on the function of biological photosensors

    The research groups of Janne Ihalainen (University of Jyväskylä) and Sebastian Westenhoff (University of Gothenburg) have clarified how the atom structure of bacterial red light photosensors changes when sensing light. The research reveals structural changes in phytochrome protein when illuminated. more

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White papers Proteins

  • Enzymatic Protein Digest for Mass-spectrometric Analysis

    The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional electrophoresis must be proteolytically cleaved in the course of sample preparation for identification and characterization. A reproducible cleavage pattern of digested proteins is a prerequisite for the unambiguous identification of these proteins by mass spectrometry (MS). more

  • Measurement of Nano particles and Proteins

    Colloid emulsions in their native environment require particle size determination in high concentration since there are concerns about changes in sample morphology upon dilution eg break up of aggregates. For proteins and other samples with very low concentration and for weakly scattering samples high sensitivity is required. New technologies offer both. more

  • Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

    Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

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