ISOLUTE^® SLE+ Supported Liquid Extraction Plates

ISOLUTE^® SLE+ Supported Liquid Extraction plates offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, extracting up to 400 µL aqueous sample. ISOLUTE SLE+ plates provide high analyte recoveries, eliminate emulsion formation, and cut sample preparation time in half.

Efficient extraction
Supported-liquid extraction mechanism is very efficient, delivering higher analyte recoveries and cleaner extracts than the equivalent LLE method.

No emulsion formation
Emulsions cannot form because the sample and water immiscible extraction solvent are never in direct contact, preventing contamination and maximizing analyte recovery.

Easy to automate
ISOLUTE SLE+ plates provide an easy-to-automate alternative to traditional liquid-liquid extraction. No manual ‘off-line’ steps (capping/mixing/centrifuge/de-capping) required. All procedural steps can be fully automated with no manual intervention necessary.

Good flow characteristics
The ISOLUTE SLE+ plate is packed with an optimized grade of diatomaceous earth, providing reproducible flow characteristics from well-to-well. Aqueous samples and extraction solvents load evenly across the plate, an important feature for automated sample preparation procedures, where well blockage can lead to loss of valuable samples.

Higher analyte recoveries
High extraction efficiency and elimination of emulsions provide higher analyte recoveries and lower detection limits.

Transferable methods
The water immiscible extraction solvents used in LLE can also be used for ISOLUTE SLE+ procedures. Sample pretreatment conditions are also the same, meaning existing LLE methods are easily transferable to ISOLUTE SLE+, reducing method development time.

Features and Benefits

• Automation friendly procedure improves productivity – no off-line steps required
• Efficient extraction process maximizes analyte recovery compared to LLE
• Excellent flow characteristics improve reproducibility
• Minimal method development. ISOLUTE SLE+ uses the same solvent systems as LLE allowing direct transfer of methods
• Same clean extracts as LLE – reduced ion suppression leads to improved signal to noise
• Process manually using VacMaster™-96 Sample Processing Manifold or using automated liquid handling systems equipped with a vacuum manifold
• Fixed well plate format is compatible with all common liquid handling systems
• The ISOLUTE SLE+ has multiple uses as for pollution testing, environmental testing and poisons as well as forensics and the testing for chemicals and drugs within bodily fluids.

SLE in 96-well Plates
Each plate is made up of 96 individual wells, each containing a modified form of diatomaceous earth.

Options
ISOLUTE SLE+ is available in the industry standard 2 mL fixed well ‘SPE’ plate format and is compatible with all commercially available automated liquid handling systems.

Two ISOLUTE SLE+ plate sizes are available. The 200 mg plate allows a total aqueous sample load of up to 200 µL; the 400 mg plate allows aqueous sample load of 200 – 400 µL.

For further information please visit our product website, download our product brochure or get in contact with us!