Topic PCR

Products

ECO High-End Real-time PCR Cycler

The Eco Real-Time PCR starts a new dimension in real-time PCR. It is an easy-to-use, affordable, personal instrument. Read more! more

PIROs - your personal pipetting robot

PIRO is very easy to program and can be used for many liquid handling needs from simple liquid transfers to highly complex applications more

Products PCR

ECO High-End Real-time PCR Cycler

The Eco Real-Time PCR starts a new dimension in real-time PCR. It is an easy-to-use, affordable, personal instrument. Read more! more

News PCR

• analytica presents biotechnology highlights

  From molecular-biology techniques to forensic DNA analysis: from April 17 – 20 analytica features in-depth information about the latest methods and techniques in laboratory technology, analysis and biotechnology. One of the five halls, hall A3, is devoted entirely to the biotech industry. The topic more

• QIAGEN and Max Planck Institute for Infection Biology collaborate to develop assay for active TB risk in individuals with latent infection

  QIAGEN N.V. and the Max Planck Institute for Infection Biology (MPIIB), Department of Immunology have announced a new collaboration to develop a molecular diagnostic test to assess the risk of an individual with latent tuberculosis (TB) developing active TB disease during their lifetime. Financial t more

• Lab21 Announces Positive Results for Aspergillus Detection in Critical Care and Haematology Patients

  Lab21 Limited has announced the completion of an evaluation of a commercial PCR test, its MycAssayTM Aspergillus assay, to detect invasive Aspergillosis (IA) in a mixed population of 158 patients with underlying haematological or critical illness. The prospective evaluation was performed by Professo more

White papers PCR

• High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests
  

  For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic techniques for quantitative monitoring of these biomarkers are required. In this work, the development of a high sensitive assay for follistatin based on the Imperacer® technology is described. The quantification limit in human serum was found at 60 pg/ml in only 6 µl sample volume. With this initial assay we demonstrate the first application in a set of analytical tests for a high sensitive myostatin pathway related profiling. more

• Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent
  

  Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

• Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System
  

  One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleo­tides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR). Ideally, a real-time multiplex PCR should be able to detect, differentiate, and provide a quantitative result for many different targets without a single target influencing the detection of one of the others (cross-talk) and without loss of sensitivity. It is evident that due to the limited number of fluorophoric labels available and the significant overlap in their emission spectra, quantification of multiplex reaction products is difficult and often not possible for more than two targets. more

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