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News pathology

• China Medical Technologies and Leica Microsystems Announce Collaboration

  China Medical Technologies, Inc. (CMED) and Leica Biosystems, a division of Leica Microsystems, announced that they have established a sales, research and development collaboration to co-develop and market automated FISH kits to be used on the Leica BOND system. CMED will sell the Automated FISH Kit more

• Brain imaging gives new insight into mental disorders

  A new kind of psychiatry built on objective measures derived from functional magnetic resonance imaging (or fMRI) of the brain performed while patients play economic games could provide new insight into the diagnosis and, eventually, treatment of mental disorders, said researchers from Baylor Colleg more

• New tagging technique enhances view of living cells

  Scientists hoping to understand how cells work may get a boost from a new technique to tag and image proteins within living mammalian cells. The new technique, developed by a research team led by University of Illinois at Chicago assistant professor of chemistry Lawrence Miller, provides the cleare more

White papers pathology

• Superiority of FuGENE® HD Transfection Reagent in Minimizing Non-Specific Cellular Interferon Responses
  

  Many viruses, including human cytomegalovirus (HCMV), encode for genes that can modulate or interfere with the ability of cells to mount an effective antiviral response to infection. Transfection of viral genes into cultures of mammalian cells is an important research tool to evaluate the function of viral gene products. We utilized this approach to identify viral genes involved in modulation of interferon (IFN)-mediated cell signaling pathways. more

• MagNA Pure Compact RNA Isolation Kit: Isolation of High-Quality Total RNA from a Broad Range of Sample Material
  

  The MagNA Pure Compact Instrument together with the new MagNA Pure Compact RNA Isolation Kit can be used for automated isolation of total RNA from blood, blood cells, tissue, and cultured cells. The MagNA Pure Compact System perfectly fits into Roche Applied Science's integrated solution for real-time PCR: The MagNA Lyser Instrument, Universal ProbeLibrary, Transcriptor First Strand cDNA Synthesis Kit and FastStart TaqMan® Probe Master. more

• cDNA Synthesis with the Transcriptor First Strand cDNA Synthesis Kit from Formalin-Fixed Paraffin-Embedded Tissue (FFPET) Samples
  

  Partial degradation of RNA is a common problem when working with FFPET samples. Semi-­quantitative RT-PCR assays amplifying the 5´- and 3´-end sequences of the housekeeping gene b-actin present a solution: calculating the ratio of the 3´- to 5´-amplicon allows ­assessment of RNA integrity. An important step for reproducible quantification is the reverse ­transcription reaction. The ­applicability of the new Transcriptor First Strand cDNA Synthesis Kit to partially degraded FFPET RNA was evaluated. The new kit is compared with the widely used First Strand cDNA Synthesis Kit for RT-PCR (AMV). Under modified reaction ­conditions (temperature, time) the kit reversely transcribes the FFPET samples with high ­efficiency. more

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