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Three wavelengths will do

Practical approach for assessing the melanin and blood content of the skin

24-Apr-2015

Many factors can change skin pigmentation, including aging, exposure to UV light, certain drugs, as well as certain diseases. A simple technique for measuring skin pigmentation could be a helpful tool for research and diagnostics. The same goes for measuring the skin blood content. Alteration of ...

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Trapping and watching motile cells

A new approach enables rapid characterization of living suspension cells in 4 dimensions

01-Apr-2015

Optical imaging of highly motile cells or cells in suspension, such as bacterial systems, yeast cells, and immune cells, is a challenging task, in many cases it is just not possible. The human immunodeficiency virus (HIV), for example, can spread very efficiently from an infected T cell to an ...

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New Endoscopic Technique

Flexible wide-field endoscope provides label-free contrast of tissue based on fluorescence lifetime imaging of tissue autofluorescence

11-Feb-2015

Fluorescence lifetime imaging (FLIM) of tissue autofluorescence has been shown to provide labelfree contrast between different types and states of tissue. Clinical FLIM can combine lifetime contrast with morphological information to provide a direct comparison between different spatial regions – ...

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Measuring Skin Rejuvenescence

In vivo and non-invasive: Collagen remodeling after micro-ablative fractional laser resurfacing can be monitored by two-photon microscopy.

25-Nov-2014

Many people in the Western World consider it as a social need to hide the effects of aging. For this purpose, different cutaneous rejuvenation treatments have been developed, including a laser-based technique, known as laser resurfacing. Skin irradiation with high-power pulsed laser light induces ...

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Exploiting synergies

12-Dec-2013

Multimodal nonlinear microscopy has matured to a key imaging modalities in life science and biomedicine. It offers label-free visualization of tissue structure and chemical composition, high depth penetration, intrinsic 3D sectioning, diffraction limited resolution and low phototoxicity. In the ...

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Vibrational molecular pathology

11-Dec-2013

IR and Raman spectral imaging can distinguish between tissue types, disease types and stages, and even identify the primary tumors from spectral patterns observed in metastatic cells. Furthermore, these techniques can be used in the detection of circulating tumor cells. Spectral histopathology ...

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Raman pixel by pixel

15-Aug-2013

Raman spectroscopy provides molecular specificity through spectrally-resolved measurement of the inelastic scattering under monochromatic excitation. In the context of microscopy, it may serve as label-free cell imaging, providing structural information. However, the very low cross-section of ...

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A well-matched couple

A new setup for hybrid photoacoustic and ultrasound imaging with optical ultrasound detection provides perfectly co-registered dual-modality section images

14-Jun-2013

Photoacoustic (PA) imaging is a method to visualize structures with optical contrast in biological tissue. Despite the strong optical scattering in tissue, high resolution images in the visible and near infrared spectral range can be obtained. The method is based on sound waves which are ...

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Dying Brightly

Fluorescence lights up cells programmed to die

28-Jan-2013

Programmed cell death, or apoptosis, occurs tens of millions of times every day in every human body. Researchers in South Korea have devised an easy method to detect apoptotic cells by fluorescence, as they report in Chemistry—An Asian Journal. Their method makes it easier to detect improper ...

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A deep look into the body with Raman spectroscopy

24-Jan-2013

Raman spectroscopy has recently undergone major advances in the area of deep non-invasive characterisation of biological tissues. The progress stems from the develop­ment of spatially offset Raman spectroscopy (SORS) and renaissance of transmission Raman spectroscopy permitting the assessment of ...

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