Promega and BASF have jointly developed an alternative method to animal studies that can reliably detect the allergenic potential of substances. Using a newly developed cell line, the reaction of skin cells to allergenic substances can now be demonstrated in the test tube. Allergic skin rea ... more
Promega and Leica Microsystems Sign Agreement to Develop Fluorescent Ligands Enabling Live Cell Imaging at the Sub 100 nm Range
Promega Corp. announced that it has signed an agreement with Leica Microsystems to enable fluorescent imaging of proteins in live cells. Under the terms of the agreement, Promega will develop fluorescent ligands for its HaloTag fusion proteins, that will be used to label proteins within live cells so that they can be visualized with Leica's TCS STED (stimulated emission depletion) superresolution microscope. Developed in the laboratory of Dr. Stefan Hell of the Max-Planck Institute in Germany, STED microscopy enables the investigation of structural details below the 100nm range.
Commonly used fluorescent proteins such as GFP are not optimal for use with high-powered STED technology as they are prone to photobleaching and lack appropriate spectral properties. In addition, alternative methods of labeling proteins with fluorescent antibodies are only applicable for fixed cells. Therefore, the agreement between Promega and Leica Microsystems will enable imaging of proteins within live cells at sub 100nm range.
Promega has been working with Leica Microsystems since 2006 to develop novel ways to label proteins within cells using its proprietary HaloTag® technology.
- Stefan Hell
- live cell imaging
- fusion proteins
- fluorescent proteins
Markus Lusser is the new President and Director of Leica Microsystems, headquartered in Wetzlar, Germany. This appointment became effective July 1, 2015. He succeeds Andries Peter Jan van den Broek who has left the company. Before joining Leica Microsystems, Markus Lusser was Vice President ... more
The Scientist magazine has chosen the Leica TCS SP8 STED 3X as one of the Top 10 Innovations 2014 that will change the way life scientists work. An independent jury of experts from science and industry selected the latest generation of Leica Microsystems’ super-resolution microscopes. This ... more
On Monday, October 20, 2014, Yale University West Campus will open the doors to a new microscopy Center of Excellence made possible through a partnership with Leica Microsystems. Housed in a newly created core facility, the Leica Center of Excellence at Yale West Campus will provide scienti ... more
- White papers
- 1Mechanism involved in novel drug design with potential to treat tuberculosis
- 2Nanoscale virus features reconstructed from correlations of scattered X-rays
- 3New antibiotic resistance genes found
- 4Neutrons observe vitamin B6-dependent enzyme activity useful for drug development
- 5Eurofins acquires food testing laboratory Institut Nehring
- 6A detailed look at 2-D structure of turbulence in tokamaks
- 7Pioneering discovery of an odor-detecting receptor enhancer
- 8How rough microparticles can cause big problems
- 9Initiative taps scientists to create atlas of cells in human spinal cord
- 10Proteins may prevent dysfunction and disease by relaxing