11-02-2007: Roche and Eppendorf announced that they have entered into a license agreement for use of the melting curve analysis. Under the terms of the agreement, Eppendorf obtains from Roche a non-exclusive license to use, develop and sell products based on Melting Curve Analysis; excluded from the rights is the use in human and veterinary in vitro diagnostics. Financial terms of the agreement were not disclosed.
The melting curve analysis is a technique for analysis of PCR products. After PCR, the resulting PCR product is slowly heated. Each double-stranded DNA has its own specific melting temperature (Tm), which is defined as the temperature at which 50% of the DNA becomes single stranded. By measurement of Tm, the DNA can be easily identified.
"We regard the licensing of the melting curve analysis technique as an important contribution to further developing this technology in the life science field," states Manfred Baier, Head of Roche Applied Science, a business area of Roche Diagnostics.
Roche Researchers Sequence Complete Genome of Cynomolgus Monkey
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Roche Diagnostics Supports European Union’s DETECTIVE Project to Reduce Animal Testing
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Deadly E. Coli Strain Sequenced with Roche GS Junior System by HPA Scientists
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Enzymatic Protein Digest for Mass-spectrometric Analysis
The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional elect ... more
Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent
Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. ... more
Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System
One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleotides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR). Ideally, a ... more
Manager Supplier Quality Management (M/F)
At Roche, about 80,000 people across 150 countries are pushing back the frontiers of healthcare. Working together, we’ve become one of the world’s leading research-focused healthcare groups. Our success is built on innovation, curiosity and diversity, and on seeing each other’s differences ... more
Eppendorf continues on course for growth
In the Life Science markets conditions improved with the economic recovery in the Financial Year 2010. While many companies made up their losses from the previous year which were due to the economic crisis, Eppendorf was able to consolidate its market position again during the reporting yea ... more
Eppendorf Centrifuge 5424 R wins a ‘red dot award: product design 2011’
Eppendorf AG celebrates success in a leading global design competition which attracted 4,433 entries from 1,700 companies in over 60 nations. Centrifuge 5424 R, a 24-place microcentrifuge, has been honoured for design excellence in the life science and medicine category of ‘red dot award: p ... more
Eppendorf Young Investigator Award 2008 for Dr. Simon Boulton
Dr. Simon Boulton of the London Research Institute (Clare Hall Laboratories, South Mimms, Hertfordshire) won the Eppendorf Young Investigator Award for his groundbreaking work on SPAR1, a newly discovered helicase. SPAR1 plays an important, controlling role in the repair of double-stranded ... more
The new 338-page Eppendorf Catalogue is now available detailing a broad range of instruments and consumables specific to application areas. Request your FREE personal copy today! more
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