Adenoviral infections have a mild disease progression in healthy people, but it can be dangerous for immunocompromised people. If a patient is infected with the virus and gets a bacterial infection on top of it, it can lead to an excessive inflammatory response known as a cytokine storm, an ... more
Micro-Copier for Genome AnalysisNew method holds promise to advance personalised medicine
The DNA sequences are replicated in picowell arrays (bottom) and transferred on microscopic slides (top).
The scientists Jochen Hoffmann, Dr. Guenther Roth, and Prof. Dr. Roland Zengerle from the Department of Microsystems Engineering (IMTEK) at the University of Freiburg can copy simultaneously 100.000 different DNA sequences in a so called picowell array that has the size of a one cent coin.
The picowell array is a chip with 100.000 wells on which the DNA sequences are dispersed in order to achieve a statistical distribution of one sequence per well. All wells are then covered with one microscopic slide and the DNA sequences are replicated by polymerase chain reaction (PCR) technique. The resulting DNA copies bind to the miscroscopic slide at exactly the position of the original DNA sequence. The DNA copies can be decoded by next-generation sequencing systems in the wells of the array. The microscopic slides can be used, similar to DNA microarrays, for the rapid and cost-effective analysis of entire genomes – without the need for other equipment or additional transfer steps.
Such genome analyses allow insights into the regulatory systems of an organism. For example the genome analysis of a tumour cell can reveal which of its signal paths are defective. This knowledge in the context of “personalised medicine” can help to develop a tailored therapy for a specific patient group.
The project was supported by the “Program for Excellency in Science and Technology” as part of a grant from the Hans L- Merkle-Stiftung. The Robert Bosch GmbH and the Association for the Promotion of Science and Humanities in Germany also supported the project.
The topic is now further developed in the Lab-on-a-Chip research groups “Arrays” of Jochen Hoffmann and “Assays” of Dr. Guenther Roth. Lab-on-a-chip is a main research topic in cooperation with the Laboratory for MEMS Applications and the Institut fuer Mikro- und Informationstechnik der Hahn-Schickard-Gesellschaft (HSG-IMIT). The research groups are based on the campus of the Faculty for Engineering.
- next generation sequencing
Personalized drug therapy, adjusting the dose, dosage intervals, and the duration of treatment to fit individual patients' needs, are getting more and more important. Frequently, medications are dosed in such a way that each patient receives the same standardized amount of a certain drug. T ... more
Researchers from Freiburg have developed a sensor platform that quantifies antibiotics in human blood within minutes A team of researchers from the University of Freiburg has developed a system inspired by biology that can detect several different antibiotics in human blood or other fluids ... more
- 1A sensor for the most important human cancer gene
- 2Scientists capture colliding organic nanoparticles on video for first time
- 3Eurofins expands its presence in India
- 4Symcel secures €3.6 million Horizon 2020 Phase II grant
- 5Manganese-based MRI contrast agent may be safer alternative to gadolinium-based agents
- 6Hummingbird Diagnostics and Saarland University Collaborate in Early Disease Detection Based on Molecular Markers
- 7New imaging technique peers inside living cells
- 8Computer program finds new uses for old drugs
- 9Clever use of mirrors boosts performance of light-sheet microscope
- 10Water world