Developing tiny probes for research and brain-related medical applications – that is the goal of the project NeuroSeeker, launched in February 2013 with a kick-off event in Leuven. The interdisciplinary project pools the expertise of ten partner institutions from Europe and Canada. The Univ ... more
Micro-Copier for Genome Analysis
New method holds promise to advance personalised medicine
The DNA sequences are replicated in picowell arrays (bottom) and transferred on microscopic slides (top).
02-08-2012: The scientists Jochen Hoffmann, Dr. Guenther Roth, and Prof. Dr. Roland Zengerle from the Department of Microsystems Engineering (IMTEK) at the University of Freiburg can copy simultaneously 100.000 different DNA sequences in a so called picowell array that has the size of a one cent coin.
The picowell array is a chip with 100.000 wells on which the DNA sequences are dispersed in order to achieve a statistical distribution of one sequence per well. All wells are then covered with one microscopic slide and the DNA sequences are replicated by polymerase chain reaction (PCR) technique. The resulting DNA copies bind to the miscroscopic slide at exactly the position of the original DNA sequence. The DNA copies can be decoded by next-generation sequencing systems in the wells of the array. The microscopic slides can be used, similar to DNA microarrays, for the rapid and cost-effective analysis of entire genomes – without the need for other equipment or additional transfer steps.
Such genome analyses allow insights into the regulatory systems of an organism. For example the genome analysis of a tumour cell can reveal which of its signal paths are defective. This knowledge in the context of “personalised medicine” can help to develop a tailored therapy for a specific patient group.
The project was supported by the “Program for Excellency in Science and Technology” as part of a grant from the Hans L- Merkle-Stiftung. The Robert Bosch GmbH and the Association for the Promotion of Science and Humanities in Germany also supported the project.
The topic is now further developed in the Lab-on-a-Chip research groups “Arrays” of Jochen Hoffmann and “Assays” of Dr. Guenther Roth. Lab-on-a-chip is a main research topic in cooperation with the Laboratory for MEMS Applications and the Institut fuer Mikro- und Informationstechnik der Hahn-Schickard-Gesellschaft (HSG-IMIT). The research groups are based on the campus of the Faculty for Engineering.
J. Hoffmann, H.M. Trotter, F. von Stetten, R.Zengerle and G.Roth: Solid-phase PCR in a picowell array for immobilizing and arraying 100,000 PCR products to a microscope slide, Lab Chip, 2012
Contact / Request information
Request further information free of charge:
- next generation sequencing
An estimated 220 million people become infected with malaria each year. The disease is often lethal – particularly in tropical developing countries with insufficient health care services. The infected suffer from a high fever. As this is also the case with other germs, however, it is import ... more
The scientists Jochen Hoffmann, Dr. Guenther Roth, and Prof. Dr. Roland Zengerle from the Department of Microsystems Engineering (IMTEK) at the University of Freiburg can copy simultaneously 100.000 different DNA sequences in a so called picowell array that has the size of a one cent coin. ... more
- 1Thermo Fisher Scientific to Acquire Life Technologies Corporation
- 2Schleicher & Schuell has been purchased by Whatman plc
- 3analytica Vietnam 2013 – Platform for innovations
- 4Inspecting water quality with Raman spectroscopy
- 5LIMS/LIS Market and POCT Supplement
- 6X-ray analysis could boost legumes, thus reducing fertilizer pollution
- 7Abbott Completes Acquisition of Advanced Medical Optics
- 8MDS Sciex Opens Manufacturing Facility in Singapore
- 9Troubling levels of toxic metals found in lipstick
- 10Analytik Jena announces forecast for financial year 2012/2013
- Carl Zeiss Meditec improves its results in first six months of financial year 2012/2013
- Evonik selects OPX Biotechnologies for joint development of bio-based chemicals
- ZEISS presents half-year financial figures
- Analytik Jena to develop products for sepsis diagnostics
- Analytik Jena AG establishes subsidiary in Thailand