Using 3D electron microscopy, structural biologists from the University of Zurich succeeded in elucidating the architecture of the lamina of the cell nucleus at molecular resolution for the first time. This scaffold stabilizes the cell nucleus in higher eukaryotes and is involved in organiz ... more
A giant step in a miniature world: UZH researcher measures the electrical charge of nano particles
Cross-section through two chip-sized glass plates in which a nano particle is trapped in an energy hole (or “potential well” to use the scientific term). The colored fields show the different charges in the electrostatic field. The red zone signifies a very low charge, while the blue edges have a strong charge.
Nano particles are a millionth of a millimeter in size, making them invisible to the human eye. Unless, that is, they are under the microscope of Prof. Madhavi Krishnan, a biophysicist at the University of Zurich. Prof. Krishnan has developed a new method that measures not only the size of the particles but also their electrostatic charge. Up until now it has not been possible to determine the charge of the particles directly. This unique method, which is the first of its kind in the world, is just as important for the manufacture of drugs as in basic research. The process has now been introduced for the first time in the «Nature Nanotechnology» journal.
In order to observe the individual particles in a solution, Prof. Madhavi Krishnan and her co-workers «entice» each particle into an «electrostatic trap». It works like this: between two glass plates the size of a chip, the researchers create thousands of round energy holes. The trick is that these holes have just a weak electrostatic charge. The scientists than add a drop of the solution to the plates, whereupon each particle falls into an energy hole and remains trapped there. But the particles do not remain motionless in their trap. Instead, molecules in the solution collide with them continuously, causing the particles to move in a circular motion. «We measure these movements, and are then able to determine the charge of each individual particle», explains Prof. Madhavi Krishnan.
Put simply, particles with just a small charge make large circular movements in their traps, while those with a high charge move in small circles. This phenomenon can be compared to that of a light-weight ball which, when thrown, travels further than a heavy one. The US physicist Robert A. Millikan used a similar method 100 years ago in his oil drop experiment to determine the velocity of electrically charged oil drops. In 1923, he received the Nobel Prize in physics in recognition of his achievements. «But he examined the drops in a vacuum», Prof. Krishnan explains. «We on the other hand are examining nano particles in a solution which itself influences the properties of the particles».
Electrostatic charge of «nano drugs packages»
For all solutions manufactured industrially, the electrical charge of the nano particles contained therein is also of primary interest, because it is the electrical charge that allows a fluid solution to remain stable and not to develop a lumpy consistency. «With our new method, we get a picture of the entire suspension along with all of the particles contained in it», emphasizes Prof. Madhavi Krishnan. A suspension is a fluid in which miniscule particles or drops are finely distributed, for example in milk, blood, various paints, cosmetics, vaccines and numerous pharmaceuticals. «The charge of the particles plays a major role in this», the Zurich-based scientist tells us.
One example is the manufacture of medicines that have to be administered in precise doses over a longer period using drug-delivery systems. In this context, nano particles act as «packages» that transport the drugs to where they need to take effect. Very often, it is their electrical charge that allows them to pass through tissue and cell membranes in the body unobstructed and so to take effect. «That’s why it is so important to be able to measure their charge. So far most of the results obtained have been imprecise», the researcher tells us.
«The new method allows us to even measure in real-time a change in the charge of a single entity», adds Prof. Madhavi Krishnan. «This is particularly exciting for basic research and has never before been possible». This is because changes in charge play a role in all bodily reactions, whether in proteins, large molecules such as the DNA double helix, where genetic make-up is encoded, or cell organelles. «We’re examining how material works in the field of millionths of a millimeter».
Researchers from UZH and Eawag have used “environmental DNA” to determine the biodiversity of a river. Previously, this involved collecting and identifying all the organisms living in it. Using environmental DNA, however, not only is it possible to characterize the river’s biodiversity, but ... more
The congenital disease favism causes sickness and even jaundice in patients after they consume beans. The culprit is a particular enzyme deficiency, which destroys the red blood cells. Scientists from the University Children’s Hospital Zurich have now discovered that, in the event of a seve ... more
- Cancer diagnosis with a breath test
- Particle accelerator for the European XFEL X-ray laser operational
- 'Twist and shine': Development of a new photoluminescent sensor material
- Autonomous sensor could aid in early detection of urinary tract infection
- One step closer to crack the mystery of bacterial adaptation to antibiotics
- Cracking the code of Huntington's disease
- New optical nanosensor improves brain mapping accuracy, opens way for more applications
- Molecular structure of the cell nucleoskeleton revealed for the first time
- Screening the most useful nanoparticles for medicine
- Tecan appoints Klaus Lun Head of the Life Sciences Business division