It may soon be easier to protect consumers from dangerous condiments thanks to an electrochemical method developed by Chinese scientists that can spot the toxic azo dye, Orange II. They were able to spot the dye at nanomolar concentrations in ketchup and chilli products using the sensor. O ... more
Visualising DNA sequences
20-08-2010: A new, fast way to analyse DNA could be used to sequence the genomes of viruses and in the future help tackle genetic disorders such as schizophrenia and congenital heart defects.
Robert Neely and colleagues have used a DNA methyltransferase enzyme to label the 5’-GCGC-3’ DNA sequences with a fluorescent marker. Immobilising and stretching the DNA on a surface then produces a unique and reproducible pattern when combined with the markers. The result is a ‘fluorocode’ - a simple description of the DNA sequence, which can be read and analyzed like a barcode. DNA barcodes using fluorescent tagging can be read quickly as labelled samples pass a detector, but Neely’s fluorocode gives significantly enhanced resolution and uses a much smaller number of DNA molecules.
Current DNA sequencing methods are able to sequence short regions of the genome. Regions that are either duplicated or deleted relative to a reference genome are an important cause of structural variation in the human genome with links to a variety of genetic disorders. But using current sequencing methods, studying these repeats is time consuming and labour intensive.
Original publication: Robert K. Neely, Peter Dedecker, Jun-ichi Hotta, Giedre, Urbanaviciute Saulius Klimašauskas and Johan Hofkens, Chemical Science., 2010.
Contact / Request information
Request further information free of charge:
CB4 0WF Cambridge
Chloride ions are central to many biological processes and measuring the concentration of chloride ions in biological fluids can help diagnose a number of diseases, including cystic fibrosis. Scientists from the US have synthesised a squaraine rotaxane which can detect chloride ions via col ... more
Using Fourier transform infrared spectroscopy (FTIR), scientists from Germany were able to differentiate between carcinoma, normal tissue and necrosis, and determine the primary tumour of brain metastasis. Using support vector machines and a training set of known primary tumours, it was pos ... more
Oude Markt 13
Despite advanced genome technologies, it remains a major challenge to identify small variations between the hereditary material (DNA) of different individuals. VIB scientists connected to the K.U.Leuven and the University of Antwerp describe in Nature Biotechnology a method that makes it po ... more
Stable dyes with sharp absorption and fluorescence emission bands in the red or NIR region of the spectrum, combined with high molar absorption coefficients and high fluorescence quantum yields, may find extensive use in many different fields, such as optical engineering, analytical chemist ... more
A new, fast way to analyse DNA could be used to sequence the genomes of viruses and in the future help tackle genetic disorders such as schizophrenia and congenital heart defects. Robert Neely and colleagues have used a DNA methyltransferase enzyme to label the 5’-GCGC-3’ DNA sequences wi ... more
- 1Thermo Fisher Scientific to Acquire Life Technologies Corporation
- 2analytica Vietnam 2013 – Platform for innovations
- 3Schleicher & Schuell has been purchased by Whatman plc
- 4Inspecting water quality with Raman spectroscopy
- 5LIMS/LIS Market and POCT Supplement
- 6X-ray analysis could boost legumes, thus reducing fertilizer pollution
- 7Abbott Completes Acquisition of Advanced Medical Optics
- 8Thermo Fisher Scientific Scientist Dr. Alexander Makarov Receives Inaugural HUPO Science and Technology Award
- 9Analytik Jena announces forecast for financial year 2012/2013
- 10MDS Sciex Opens Manufacturing Facility in Singapore
- Chemistry breakthrough sheds new light on illness and health
- Detecting harmful dye used to make ketchup red
- Targeted screening for C. difficile upon hospital admission could potentiall ...
- Troubling levels of toxic metals found in lipstick
- Core facilities: Widening access to research instrumentation
- Ghent University and MDxHealth Collaborate to Establish a Center in Pharmaco (Epi)genomics
- Pacific Biosciences and Imec Announce Collaboration to Develop Advanced Microchips for Single Molecule Sequencing Applications
- Trinean appoints new CEO and secures 2.7 million euro financing
- Pepric, a spin-off of imec, successfully concluded a EURO 1.4 million capital round.
- VIB scientists present method to reduce error rate in detecting genomic variants by short-read sequencing