Many factors can change skin pigmentation, including aging, exposure to UV light, certain drugs, as well as certain diseases. A simple technique for measuring skin pigmentation could be a helpful tool for research and diagnostics. The same goes for measuring the skin blood content. Alterati ... more
Written in RedRed-Emitting Dyes for Optical Microscopy and Nanoscopy
Far-field optical nanoscopy methods, especially STED (stimulated emission depletion), pose very strict and, at times, contradictory requirements on the utilized fluorescent markers. Photostable fluorescent dyes that absorb in the red optical region are indispensable as labels for various micro- and nanoscopic studies (e.g., with commercially available STED microscopes). Despite many attempts to design novel and improved red-emitting dyes, the number of compounds that perform satisfactorily in fluorescence-based microscopy is still limited. Because of this, a great deal of research is being carried out by a large multidisciplinary team headed by Prof. Stefan W. Hell at the Department of NanoBiophotonics in Max Planck Institute for Biophysical Chemistry (Göttingen). In their recent paper published in the European Journal of Organic Chemistry the team describes a general synthetic route to new improved carbopyronine dyes and their performance in confocal and STED microscopy.
The new dyes have large fluorescence quantum yields, high water solubility, and the required positions of the absorption and emission bands in the red. The chemists came up with a synthetically feasible structural scaffold with functional groups that can be varied in the final steps of the synthesis or even in the resulting fluorescent dye to fit a given task. According to Dr. Kirill Kolmakov, who performed the synthesis, his “table book” contained a dissertation and articles and patents by Prof. K. Drexhage and co-workers, whose contribution to the synthesis of carbopyronines is fundamental. However, the synthetic approach presented in their article is by far more flexible and improved. In particular, it starts from one simple precursor and utilizes a minimum amount of protecting groups. The key feature of the general strategy described therein is the interplay of certain protecting groups. Protecting groups will take an even more important part in the design and synthesis of caged carbopyronines and rhodamines that emit in the far-red spectral region. Besides the interesting chemistry, the team demonstrates that the performance of a dye in confocal microscopy and under STED conditions does not necessarily correlate. Dr. Kolmakov thus emphasizes that for their future research work, they will have to reconsider some of their old views on the “ideal” STED dye. All these make the primary article a good example of teamwork that is strategically sound, brilliantly planned, and perfectly delivered.
Original publication: Vladimir N. Belov et al.; "A Versatile Route to Red-Emitting Carbopyronine Dyes for Optical Microscopy and Nanoscopy"; European Journal of Organic Chemistry 2010, No. 19, 3593–3610.
Optical imaging of highly motile cells or cells in suspension, such as bacterial systems, yeast cells, and immune cells, is a challenging task, in many cases it is just not possible. The human immunodeficiency virus (HIV), for example, can spread very efficiently from an infected T cell to ... more
Fluorescence lifetime imaging (FLIM) of tissue autofluorescence has been shown to provide labelfree contrast between different types and states of tissue. Clinical FLIM can combine lifetime contrast with morphological information to provide a direct comparison between different spatial regi ... more
To explore the most intricate structures of the brain in order to decipher how it functions – Stefan Hell's team of researchers at the Max Planck Institute for Biophysical Chemistry in Göttingen has made a significant step closer to this goal. Using the STED microscopy developed by Hell, th ... more
Prof. Dr. Dr. h. c. Stefan Hell of the Max Planck Institute for Biophysical Chemistry in Göttingen is to receive the 2011 Körber European Science Prize endowed with 750,000 euros for his pioneering discoveries in the field of optics. Every year, the Körber Prize is awarded to an outstanding ... more
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