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  • Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology

    MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18-25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA transcripts annotated. Although miRNAs represent a relatively abundant class of transcripts, their expression levels vary greatly among species and tissues. Less abundant miRNAs routinely fail detection using technologies such as cloning, northern hybridization and microarray analysis. Here, we present a real-time quantification method for accurate, sensitive, and economical detection of plant miRNAs based upon the stem-loop RT primer approach using an miRNA-specific primer coupled with a stem-loop specific Universal ProbeLibrary (UPL) probe. This approach represents a highly specific, sensitive, and easy-to-apply system to detect miRNAs. more

  • Metagenomics Analysis Using the Genome Sequencer FLX System

    The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing. The Genome Sequencer FLX is built upon 454 Sequencing technology that enables long read lengths and very high single-read accuracy. One application in which the technology is accelerating the field's understanding is metagenomics. more

  • Genome Sequencer 20 System: Breakthrough in a New Applications Age of Sequencing

    The Genome Sequencer 20 System is an ultra-high-throughput automated DNA sequencing system capable of carrying out and monitoring sequencing reactions in a massively parallel fashion. Since the Genome Sequencer 20 System provides a complete solution for ultra-high-throughput DNA sequencing, an individual researcher can for the first time prepare samples and sequencing reactions, generate sequence reads, and assemble genome sequence data within days. more

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Roche NimbleGen | Home

    Products Technology Support Contact News Company   Sequence Capture Enrich thousands of targeted loci from complex genomic DNA with programmable Sequence Capture microarrays for sequencing on the GS FLX Sequencer from 454 Life Sciences.   CGH Measure DNA copy number differences between a test an more

Genovoxx GmbH

GenoVoxx GmbH Company | Technology | Products | Patents | News & Events | Contact us Company Profile Genovoxx is developing a new sequencing technology based on massively parallel single molecule analysis (AnyGene.Technology TM ). Inspired by the idea of analysing complete genomes within hours, the more

Alios: Technology

Home About Us Overview Management Team Board of Directors Scientific Advisory Board Assay Development, Biochem Technology Activators of innate immunity Phosphate Protected Nucleotide Prodrugs Hyperglycosylated Type I Interferons News Room Press Releases Scientific Publications Careers Contact Contac more

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