Genetics

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  • New gene responsible for cleft lip and palate syndrome identified

    An international team led by researchers from Karolinska Institutet in Sweden has identified a new gene related to the Van der Woude syndrome, the most common syndrome with cleft lip and palate. The study is published in the scientific periodical American Journal of Human Genetics and can lead the w more

  • Neandertal genome project reaches its goal

    An international research team led by Kay Prüfer and Svante Pääbo of the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, has determined a high-quality genome sequence of a Neandertal woman. The genome allows detailed insights into the relationships and population history of t more

  • How brain microcircuits integrate information from different senses

    A new publication in the journal Neuron sheds new light onto the unknown processes on how the brain integrates the inputs from the different senses in the complex circuits formed by molecularly distinct types of nerve cells. The work was led by new Umeå University associate professor Paolo Medini. O more

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  • The Universal ProbeLibrary - a Versatile Tool for Quantitative Expression Analysis in the Zebrafish

    The teleost zebrafish is a widely used model organism for the study of developmental and disease-related processes in vertebrates, including humans. Understanding these processes at a molecular and cellular level frequently requires the estimation of gene expression levels. Quantitative real-time PCR (qPCR) is an important tool towards this goal but has, until now, been relatively neglected in zebrafish. Here, we describe the comparison of the Universal ProbeLibrary (UPL)-based qPCR to a conventional SYBR Green I-based qPCR assay using embryonic zebrafish cDNA. We found that the Universal ProbeLibrary-based assay shows equal or better performance compared with the SYBR Green I assay. more

  • Real-Time PCR Quality Control for Gene Expression

    Quantitative real-time PCR (qPCR) has become the de facto standard for nucleic acid quantification. This achievement is due in large part to its sensitivity, specificity, accuracy, large dynamic range of linear quantification, and its speed. The qPCR technology has matured to a ready-to-use commonly available method in most molecular biology laboratories. Nevertheless, the reliability of the final quantification result depends heavily on all elements in the workflow, such as the quality of the input template (integrity and absence of inhibitors), the PCR assay (specificity, efficiency, limit of detection), and normalization strategy (validated reference genes). more

  • LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study

    The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, software, and reagents) in an academic laboratory setting we scheduled a high-throughput project including gene expression (39 targets in 88 samples = 3,400 expression values) as well as genotyping applications (3,808 genotypes; 14 SNPs in 288 samples). The project, involving five technicians and two graduate students, was accomplished within 15 working days. more

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DNA Test - Genetic Testing - Gene - Biotechnology | Asper Biotech

Asper Biotech is a genetic testing company with a set of robust and efficient DNA tests About Asper Biotech Company profile Management team Contacts Career opportunities How it works Genetic testing process Ordering genetic tests Requirements for samples Required DNA quantities Shipment of the sampl more

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