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  • New sequencing reveals genetic history of tomatoes

    An international team of researchers, led by the Chinese Academy of Agricultural Sciences in Beijing, published in the journal Nature Genetics a brief genomic history of tomato breeding, based on sequencing of 360 varieties of the tomato plant. The C.M. Rick Tomato Genetics Resource Center at UC Dav more

  • Large study reveals new genetic variants that raise risk for prostate cancer

    In an analysis of genetic information among more than 87,000 men, a global team of scientists says it has found 23 new genetic variants – common differences in the genetic code -- that increase a man's risk for prostate cancer. The so-called "meta-analysis," believed to be the largest of its kind, h more

  • Gibbon genome sequenced

    An international team of researchers that includes Christian Roos, Markus Brameier and Lutz Walter from the German Primate Center (DPZ) in Göttingen, have decoded the genome of gibbons from Southeast Asia. With this, the entire genetic information of five different species of this primate family has more

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  • Real-Time PCR Quality Control for Gene Expression

    Quantitative real-time PCR (qPCR) has become the de facto standard for nucleic acid quantification. This achievement is due in large part to its sensitivity, specificity, accuracy, large dynamic range of linear quantification, and its speed. The qPCR technology has matured to a ready-to-use commonly available method in most molecular biology laboratories. Nevertheless, the reliability of the final quantification result depends heavily on all elements in the workflow, such as the quality of the input template (integrity and absence of inhibitors), the PCR assay (specificity, efficiency, limit of detection), and normalization strategy (validated reference genes). more

  • Genotypes in Smokers: Correlations with Smoking Behavior

    Smoking behavior is influenced by both genetic and environmental factors. Moreover, smoking initiation and smoking persistence have a heritability of at least 50%. A large body of work has been dedicated to associating multiple genetic markers of neurobiological pathways previously linked to addiction and smoking such as the central dopaminergic, serotoninergic, and nicotinergic pathways with different aspects of smoking behavior. In order to investigate the feasability of future genetic analyses, we genotyped 14 SNPs in 288 samples of addicted smokers using the LightCycler® 480 System with HybProbe assays. more

  • The Universal ProbeLibrary - a Versatile Tool for Quantitative Expression Analysis in the Zebrafish

    The teleost zebrafish is a widely used model organism for the study of developmental and disease-related processes in vertebrates, including humans. Understanding these processes at a molecular and cellular level frequently requires the estimation of gene expression levels. Quantitative real-time PCR (qPCR) is an important tool towards this goal but has, until now, been relatively neglected in zebrafish. Here, we describe the comparison of the Universal ProbeLibrary (UPL)-based qPCR to a conventional SYBR Green I-based qPCR assay using embryonic zebrafish cDNA. We found that the Universal ProbeLibrary-based assay shows equal or better performance compared with the SYBR Green I assay. more

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