A new, streamlined approach to genetic engineering drastically reduces the time and effort needed to insert new genes into bacteria, the workhorses of biotechnology, scientists are reporting. Published in the journal ACS Synthetic Biology, the method paves the way for more rapid development of desig more
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Fast new, 1-step genetic engineering technology
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Material screening method allows more precise control over stem cells
When it comes to delivering genes to living human tissue, the odds of success come down the molecule. The entire therapy — including the tools used to bring new genetic material into a cell — must have predictable effects. Now, a new screening process will simplify non-viral transfection, providing more
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Learning the alphabet of gene control
Scientists at Karolinska Institutet in Sweden have made a large step towards the understanding of how human genes are regulated. In a new study they identified the DNA sequences that bind to over four hundred proteins that control expression of genes. This knowledge is required to understand how dif more
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Metagenomics Analysis Using the Genome Sequencer FLX System
The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing. The Genome Sequencer FLX is built upon 454 Sequencing technology that enables long read lengths and very high single-read accuracy. One application in which the technology is accelerating the field's understanding is metagenomics. more
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Superiority of FuGENE® HD Transfection Reagent in Minimizing Non-Specific Cellular Interferon Responses
Many viruses, including human cytomegalovirus (HCMV), encode for genes that can modulate or interfere with the ability of cells to mount an effective antiviral response to infection. Transfection of viral genes into cultures of mammalian cells is an important research tool to evaluate the function of viral gene products. We utilized this approach to identify viral genes involved in modulation of interferon (IFN)-mediated cell signaling pathways. more
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Improved Performance of the LightCycler®
Real-time reverse transcription-PCR (RT-PCR) is considered the method of choice for any experiments requiring sensitive, specific, and reproducible quantification of mRNA molecules of interest. It is an essential tool for basic research, molecular medicine, and biotechnology. more
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