Topic gene expression

News gene expression

• Agilent Technologies Awards Two Integrated-Biology Research Grants

  Agilent Technologies Inc. announced the winners of the 2011 eMerging Insights grant program: Dr. Michael J. MacCoss of the University of Washington and Dr. Peter J. Park of Harvard University. Each will be awarded $75,000 toward their ongoing research on open source data-integration tools.The first more

• Roche Researchers Sequence Complete Genome of Cynomolgus Monkey

  A team of researchers from Roche, including scientists from Roche Pharma Research and Early Development and Roche NimbleGen, reported in Genome Research that they have generated the first draft genome sequence of the cynomolgus monkey (Macaca fascicularis) and developed a novel microarray design for more

• In scientific first, researchers visualize naturally occurring mRNA

  In a technique that could eventually shed light on how gene expression influences human disease, scientists at Albert Einstein College of Medicine of Yeshiva University have for the first time ever successfully visualized single molecules of naturally-occurring messenger RNA (mRNA) transcribed in li more

White papers gene expression

• Detection of NADH and NADPH with the Omega's High Speed, Full UV/Vis Absorbance Spectrometer
  

  Nicotinamide Adenine Dinucleotide (NAD+), reduced nicotinamide adenine dinucleotide (NADH), nicotinamide adenine dinucleotide phosphate (NADP+) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) have been known to play vital roles in energy metabolism, antioxidation, and reductive biosynthesis. In recent years, these co-factors have been shown to be involved in other physiological functions as well, including aging, oxidative stress, intracellular calcium homeostasis, ROS production, cell death and gene expression. more

• High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests
  

  For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic techniques for quantitative monitoring of these biomarkers are required. In this work, the development of a high sensitive assay for follistatin based on the Imperacer® technology is described. The quantification limit in human serum was found at 60 pg/ml in only 6 µl sample volume. With this initial assay we demonstrate the first application in a set of analytical tests for a high sensitive myostatin pathway related profiling. more

• Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent
  

  Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

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