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  • The Swedish initiative to map all human proteins reaches half-way point

    Scientists in Sweden marked the half way point of a major, ground-breaking initiative to map every single protein in the human body. Once complete, the Human Protein Atlas will provide scientists with data which will help detect and treat some of the world’s most serious health problems such as canc more

  • NYU Langone scientists are first to sequence the genomes of 2 ant species

    Scientists have finally sequenced the entire genome of an ant, actually two very different species of ant, and the insights gleaned from their genetic blueprints are already yielding tantalizing clues to the extraordinary social behavior of ants. A result of a collaborative research project led by D more

  • The Porcine microRNAome is revealed

    Researchers from LC Sciences LLC and a collaboration of universities have established a porcine microRNAome, a complete catalog of all microRNAs expressed in the species Sus scrofa. MicroRNAs (miRNAs) are small regulatory RNA molecules known to control a wide array of cellular functions such as gr more

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  • Quantification of BRCA1 Expression Level Using Standard RT-PCR Reagents - a Sensitive Method for the Detection of Low Amounts of Transcripts

    Mutations in the breast cancer susceptibility gene, BRCA1, occur in the majority of families with multiple members affected with breast or ovarian cancer. Women who inherit a BRCA1 mutation have 40%-80% risk of developing breast cancer or ovarian cancer. However, up to 90% of breast cancers are sporadic, and mutations in BRCA1 seem to be a rare event in sporadic breast tumors. more

  • LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study

    The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, software, and reagents) in an academic laboratory setting we scheduled a high-throughput project including gene expression (39 targets in 88 samples = 3,400 expression values) as well as genotyping applications (3,808 genotypes; 14 SNPs in 288 samples). The project, involving five technicians and two graduate students, was accomplished within 15 working days. more

  • Analysis of the DNA Methylation Patterns at the BRCA1 CpG Island

    Germ-line alterations of the BRCA1 gene confer a lifetime risk of 40% for ovarian cancers and of 40%-80% for ­breast cancers. It is likely that BRCA1 acts as a tumor suppressor gene. BRCA1 involvement in breast cancers does not seem to be restricted to familial cancers. Despite the absence of somatic mutations in the breast tissues, a down regulation of BRCA1 expression is associated with malignancy in human sporadic breast cancers. more

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