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  • The Swedish initiative to map all human proteins reaches half-way point

    Scientists in Sweden marked the half way point of a major, ground-breaking initiative to map every single protein in the human body. Once complete, the Human Protein Atlas will provide scientists with data which will help detect and treat some of the world’s most serious health problems such as canc more

  • NYU Langone scientists are first to sequence the genomes of 2 ant species

    Scientists have finally sequenced the entire genome of an ant, actually two very different species of ant, and the insights gleaned from their genetic blueprints are already yielding tantalizing clues to the extraordinary social behavior of ants. A result of a collaborative research project led by D more

  • The Porcine microRNAome is revealed

    Researchers from LC Sciences LLC and a collaboration of universities have established a porcine microRNAome, a complete catalog of all microRNAs expressed in the species Sus scrofa. MicroRNAs (miRNAs) are small regulatory RNA molecules known to control a wide array of cellular functions such as gr more

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  • Superior Transfection of Human Embryonic Stem Cells with FuGENE® HD Transfection Reagent

    Gene manipulation techniques are potentially powerful tools in many applications for human embryonic stem (ES) cell research. Controlling expression levels of specific genes may improve the efficiency of differentiation induction. Cell types of interest may be collected using marker genes and antibiotic resistance genes. Tracking of ES cells derived after engraftment could be facilitated by tracer genes. It may also be possible to selectively destroy transplanted cells. more

  • Superiority of FuGENE® HD Transfection Reagent in Minimizing Non-Specific Cellular Interferon Responses

    Many viruses, including human cytomegalovirus (HCMV), encode for genes that can modulate or interfere with the ability of cells to mount an effective antiviral response to infection. Transfection of viral genes into cultures of mammalian cells is an important research tool to evaluate the function of viral gene products. We utilized this approach to identify viral genes involved in modulation of interferon (IFN)-mediated cell signaling pathways. more

  • Epitope Mapping Using the In Vitro Rapid Translation System RTS 100

    The Rapid Translation System (RTS) represents a cell-free system that allows efficient production of protein from linear DNA sequences. This article gives a brief outline of the use of the RTS technology in linear epitope mapping and shows its promise for reducing time-consuming cloning and cell-lysis steps. more

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