Expression

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  • The Swedish initiative to map all human proteins reaches half-way point

    Scientists in Sweden marked the half way point of a major, ground-breaking initiative to map every single protein in the human body. Once complete, the Human Protein Atlas will provide scientists with data which will help detect and treat some of the world’s most serious health problems such as canc more

  • NYU Langone scientists are first to sequence the genomes of 2 ant species

    Scientists have finally sequenced the entire genome of an ant, actually two very different species of ant, and the insights gleaned from their genetic blueprints are already yielding tantalizing clues to the extraordinary social behavior of ants. A result of a collaborative research project led by D more

  • The Porcine microRNAome is revealed

    Researchers from LC Sciences LLC and a collaboration of universities have established a porcine microRNAome, a complete catalog of all microRNAs expressed in the species Sus scrofa. MicroRNAs (miRNAs) are small regulatory RNA molecules known to control a wide array of cellular functions such as gr more

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  • LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study

    The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, software, and reagents) in an academic laboratory setting we scheduled a high-throughput project including gene expression (39 targets in 88 samples = 3,400 expression values) as well as genotyping applications (3,808 genotypes; 14 SNPs in 288 samples). The project, involving five technicians and two graduate students, was accomplished within 15 working days. more

  • Fugene® HD Transfection Reagent: Superior Performance for Challenging Expression Studies

    The ability to efficiently introduce foreign molecules into cells constitutes an important tool in any laboratory and facilitates studies of gene regulation and protein function. A plethora of transfection methods have been developed for transfer of DNA into mammalian and animal cells. Those methods either utilize the physicochemical properties of the vector-carrier of the DNA or use mammalian viruses. In the first group of methods, some classic techniques are well-known, such as: calcium phosphate, DEAE-dextran, electroporation liposome-mediated delivery, direct microinjection/biolistic particle delivery, and non-liposomal multi-component reagents. Viral vector systems use both DNA (Adeno-) and RNA (Retro-, Lenti-) viruses. more

  • MagNA Pure Compact RNA Isolation Kit: Isolation of High-Quality Total RNA from a Broad Range of Sample Material

    The MagNA Pure Compact Instrument together with the new MagNA Pure Compact RNA Isolation Kit can be used for automated isolation of total RNA from blood, blood cells, tissue, and cultured cells. The MagNA Pure Compact System perfectly fits into Roche Applied Science's integrated solution for real-time PCR: The MagNA Lyser Instrument, Universal ProbeLibrary, Transcriptor First Strand cDNA Synthesis Kit and FastStart TaqMan® Probe Master. more

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