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  • The Swedish initiative to map all human proteins reaches half-way point

    Scientists in Sweden marked the half way point of a major, ground-breaking initiative to map every single protein in the human body. Once complete, the Human Protein Atlas will provide scientists with data which will help detect and treat some of the world’s most serious health problems such as canc more

  • NYU Langone scientists are first to sequence the genomes of 2 ant species

    Scientists have finally sequenced the entire genome of an ant, actually two very different species of ant, and the insights gleaned from their genetic blueprints are already yielding tantalizing clues to the extraordinary social behavior of ants. A result of a collaborative research project led by D more

  • The Porcine microRNAome is revealed

    Researchers from LC Sciences LLC and a collaboration of universities have established a porcine microRNAome, a complete catalog of all microRNAs expressed in the species Sus scrofa. MicroRNAs (miRNAs) are small regulatory RNA molecules known to control a wide array of cellular functions such as gr more

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  • LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study

    The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, software, and reagents) in an academic laboratory setting we scheduled a high-throughput project including gene expression (39 targets in 88 samples = 3,400 expression values) as well as genotyping applications (3,808 genotypes; 14 SNPs in 288 samples). The project, involving five technicians and two graduate students, was accomplished within 15 working days. more

  • Superior Transfection of Human Embryonic Stem Cells with FuGENE® HD Transfection Reagent

    Gene manipulation techniques are potentially powerful tools in many applications for human embryonic stem (ES) cell research. Controlling expression levels of specific genes may improve the efficiency of differentiation induction. Cell types of interest may be collected using marker genes and antibiotic resistance genes. Tracking of ES cells derived after engraftment could be facilitated by tracer genes. It may also be possible to selectively destroy transplanted cells. more

  • The Universal ProbeLibrary - a Versatile Tool for Quantitative Expression Analysis in the Zebrafish

    The teleost zebrafish is a widely used model organism for the study of developmental and disease-related processes in vertebrates, including humans. Understanding these processes at a molecular and cellular level frequently requires the estimation of gene expression levels. Quantitative real-time PCR (qPCR) is an important tool towards this goal but has, until now, been relatively neglected in zebrafish. Here, we describe the comparison of the Universal ProbeLibrary (UPL)-based qPCR to a conventional SYBR Green I-based qPCR assay using embryonic zebrafish cDNA. We found that the Universal ProbeLibrary-based assay shows equal or better performance compared with the SYBR Green I assay. more

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