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News enzymes

• Gamers succeed where scientists fail
  

  Gamers have solved the structure of a retrovirus enzyme whose configuration had stumped scientists for more than a decade. The gamers achieved their discovery by playing Foldit, an online game that allows players to collaborate and compete in predicting the structure of protein molecules.After scien more

• Label-free single-molecule DNA sequencing

  Microfluidics can be used to trap a single DNA–enzyme complex in its native state, without immobilisation. These studies have usually been performed by immobilising either the enzyme or the DNA on a glass slide, but this may modify their properties, and make it difficult to analyse the products. Th more

• NYU Langone scientists are first to sequence the genomes of 2 ant species

  Scientists have finally sequenced the entire genome of an ant, actually two very different species of ant, and the insights gleaned from their genetic blueprints are already yielding tantalizing clues to the extraordinary social behavior of ants. A result of a collaborative research project led by D more

White papers enzymes

• Enzymatic Protein Digest for Mass-spectrometric Analysis
  

  The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional electrophoresis must be proteolytically cleaved in the course of sample preparation for identification and characterization. A reproducible cleavage pattern of digested proteins is a prerequisite for the unambiguous identification of these proteins by mass spectrometry (MS). more

• High Pure PCR Cleanup Micro Kit - Novel Flexibility for Nucleic Acid Purification
  

  Manual nucleic acid isolation with High Pure products is long approved by the scientific community. The product portfolio includes kits for isolation of genomic RNA and DNA, plasmid DNA as well as purification of reaction products. Key goal for all products is to offer optimal solutions suited for many different sample materials in order to reduce the number of different nucleic acid isolation kits necessary in modern molecular biology labs. more

• Quantification of Zea mays mRNAs by Real-Time PCR Using the Universal ProbeLibrary
  

  The Universal ProbeLibrary (UPL) is a detection system for real-time PCR based on locked nucleic acid (LNA) probes. We tested the efficiency of the UPL for the quantification of maize transcripts on two detection platforms. The results indicate that the UPL is an attractive alternative to assays based on intercalating dyes or conventional probes. more

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