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• CyBio and Macherey-Nagel collaborate to develop applications for automated DNA/RNA preparation

  CyBio AG and Macherey-Nagel GmH Co. KG, have signed a non-exclusive collaboration for the joint development of applications to automate Macherey-NagelL kits on CyBio liquid handling instruments. The cooperation focuses on the automation of nucleic acid preparation from various biological sample mat more

• Expiry of PCR Patent Offers Exciting Growth Opportunities for Nucleic Acid Purification and Amplification Technologies

  The most widely used nucleic amplification technologies (NATs) are based on polymerase chain reaction (PCR), whose foundational patents expired in the US in March 2005. Patent expiry is expected to unlock considerable market opportunities. Smaller companies entering the lucrative clinical kits marke more

• PowderMed's PMED Gene Gun named to the Popular Science 'Best of What's New' for 2005

  PowderMed, an immunotherapeutics company focused on the development and manufacture of DNA-based vaccines for viral diseases and cancer, announced that its Particle Mediated Epidermal Delivery (PMED(TM) ) vaccine delivery device has been selected by the editors of Popular Science as one of this year more

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• Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler® 480 System
  

  One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleo­tides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same PCR reaction (multiplex PCR). Ideally, a real-time multiplex PCR should be able to detect, differentiate, and provide a quantitative result for many different targets without a single target influencing the detection of one of the others (cross-talk) and without loss of sensitivity. It is evident that due to the limited number of fluorophoric labels available and the significant overlap in their emission spectra, quantification of multiplex reaction products is difficult and often not possible for more than two targets. more

• Metagenomics Analysis Using the Genome Sequencer FLX System
  

  The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small RNA analysis, and amplicon sequencing. The Genome Sequencer FLX is built upon 454 Sequencing technology that enables long read lengths and very high single-read accuracy. One application in which the technology is accelerating the field's understanding is metagenomics. more

• Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology
  

  MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18-25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA transcripts annotated. Although miRNAs represent a relatively abundant class of transcripts, their expression levels vary greatly among species and tissues. Less abundant miRNAs routinely fail detection using technologies such as cloning, northern hybridization and microarray analysis. Here, we present a real-time quantification method for accurate, sensitive, and economical detection of plant miRNAs based upon the stem-loop RT primer approach using an miRNA-specific primer coupled with a stem-loop specific Universal ProbeLibrary (UPL) probe. This approach represents a highly specific, sensitive, and easy-to-apply system to detect miRNAs. more

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