Cell Cultures

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  • Sustainable production of pharmaceutical compounds

    European scientists have made ground-breaking discoveries for improving the efficiency of the production of pharmaceuticals through plant biotechnology. Biotechnological production offers a cost-effective and environmentally friendly alternative to the chemical synthesis of rare and complex pharmace more

  • 3D microgels 'on-demand' offer new potential for cell research

    Rather than your average bowl of Lucky Charms, these are three-dimensional cell cultures generated by an exciting new digital microfluidics platform, the results of which have been published in Nature Communications this week by researchers at the University of Toronto. The tool, which can be used t more

  • High-throughput drug screening in 3D

    Scientists in China have developed a simple microchip that enables quick and inexpensive high-throughput screening of potential drug candidates in 3D cell cultures. Scientists often use cell-based high-throughput screening in the first stage of drug design as a technique to quickly identify the most more

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  • High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests

    For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic techniques for quantitative monitoring of these biomarkers are required. In this work, the development of a high sensitive assay for follistatin based on the Imperacer® technology is described. The quantification limit in human serum was found at 60 pg/ml in only 6 µl sample volume. With this initial assay we demonstrate the first application in a set of analytical tests for a high sensitive myostatin pathway related profiling. more

  • Superior Transfection of Human Embryonic Stem Cells with FuGENE® HD Transfection Reagent

    Gene manipulation techniques are potentially powerful tools in many applications for human embryonic stem (ES) cell research. Controlling expression levels of specific genes may improve the efficiency of differentiation induction. Cell types of interest may be collected using marker genes and antibiotic resistance genes. Tracking of ES cells derived after engraftment could be facilitated by tracer genes. It may also be possible to selectively destroy transplanted cells. more

  • Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent

    Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

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