Cell Cultures

News Cell Cultures

• Top award for 3D cell culture pioneer, Stefan Przyborski, of Reinnervate Ltd

  Prof Stefan Przyborski, CSO and founder of Reinnervate Ltd, has been recognised with a top award from the Royal Society of Chemistry. The Rita and John Cornforth award 2012 recognises Prof. Przyborski’s leadership and inter-disciplinary work at the boundaries of physical chemistry and biology, devel more

• Fewer animal experiments thanks to nanosensors
  

  Countless mice, rats and rabbits die every year in the name of science – and the situation is getting worse. While German laboratories used some 2.41 million animals for scientific research in 2005, by 2009 this number had grown to 2.79 million. One third were destined for fundamental biology resear more

• Cell cultures from a machine
  

  The human genome has been decoded. Of all the puzzles it contains, though, many remain unsolved. We know that the genome provides the blueprint for various proteins, the building blocks of each and every cell. But what role do they play? Which proteins control cell division in a healthy body, for in more

White papers Cell Cultures

• High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests
  

  For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic techniques for quantitative monitoring of these biomarkers are required. In this work, the development of a high sensitive assay for follistatin based on the Imperacer® technology is described. The quantification limit in human serum was found at 60 pg/ml in only 6 µl sample volume. With this initial assay we demonstrate the first application in a set of analytical tests for a high sensitive myostatin pathway related profiling. more

• Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent
  

  Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

• Superiority of FuGENE® HD Transfection Reagent in Minimizing Non-Specific Cellular Interferon Responses
  

  Many viruses, including human cytomegalovirus (HCMV), encode for genes that can modulate or interfere with the ability of cells to mount an effective antiviral response to infection. Transfection of viral genes into cultures of mammalian cells is an important research tool to evaluate the function of viral gene products. We utilized this approach to identify viral genes involved in modulation of interferon (IFN)-mediated cell signaling pathways. more

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