Topic cell cultures

Catalogues cell cultures

Catalog 2011/2012: Cytokine & Antibody Products

PeproTech's new Catalog 2011/2012 gives an overview on which products are available for each protein more

News cell cultures

• Fewer animal experiments thanks to nanosensors
  

  Countless mice, rats and rabbits die every year in the name of science – and the situation is getting worse. While German laboratories used some 2.41 million animals for scientific research in 2005, by 2009 this number had grown to 2.79 million. One third were destined for fundamental biology resear more

• Cell cultures from a machine
  

  The human genome has been decoded. Of all the puzzles it contains, though, many remain unsolved. We know that the genome provides the blueprint for various proteins, the building blocks of each and every cell. But what role do they play? Which proteins control cell division in a healthy body, for in more

• Thermo Fisher Scientific Expands Shanghai Production Capacity

  Thermo Fisher Scientific Inc announced it has expanded its state-of-the-art manufacturing facility in Shanghai, China. This latest expansion enables Thermo Fisher to meet growing demand for its Thermo Scientific Nunc Cell Culture products including a range of tissue culture EasY Flasks, dishes, Mult more

White papers cell cultures

• High sensitive detection of follistatin by Imperacer®: an initial study on the way to analytical gene-doping tests
  

  For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological matrices and appropriate highly sensitive analytic techniques for quantitative monitoring of these biomarkers are required. In this work, the development of a high sensitive assay for follistatin based on the Imperacer® technology is described. The quantification limit in human serum was found at 60 pg/ml in only 6 µl sample volume. With this initial assay we demonstrate the first application in a set of analytical tests for a high sensitive myostatin pathway related profiling. more

• Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE® HD Transfection Reagent
  

  Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable transfection reagents and protocols are available. Few publications report successful transfection of human primary myoblasts using non-viral systems [1,2,3]. These methods include cationic lipids such as phosphono­lipids, electroporation, and a combination of liposome and adenoviral associated proteins. But transfection efficiency is low and often is a compromise between toxicity of the reagents and transfection efficiency. more

• Enabling Cancer Research with FuGENE® HD Transfection Reagent
  

  A major problem in some areas of cancer research is the difficulty of transfecting a significant number of the cells of interest, while minimizing the cytotoxic or other off-target effects of the transfection event itself. It is well-known that the use of some transfection reagents results in high levels of expression of the gene of interest in surviving cells, but also in high levels of cell death or other cellular alterations. FuGENE® HD Transfection Reagent overcomes these problems, results in high ­levels of transfection in many cancer cell lines, and thus enables researchers to conduct experiments that have previously not been possible. more

Job offers cell cultures

• Inside Sales Representatives (f/m)
  

  STEMCELL Technologies Inc is a privately-owned biotechnology company that helps power leading-edge life sciences research around the world. Scientists performing stem cell, immunology, cancer, regenerative medicine and cellular therapy research are among those who rely on our cell culture media, cel more

Websites cell cultures