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• Nanoparticles for Cultural Heritage Conservation

  The conservation of Mayan wall paintings at the archaeological site of Calakmul (Mexico) will be one on the subjects touched upon by Piero Baglioni (based at the University of Florence) in his invited lecture at the 3rd European Chemistry Congress in Nürnberg in September. In a special issue of Chem more

• Chemical element 114: A first at GSI

  At GSI Helmholtzzentrum für Schwerionenforschung, an international team of scientists succeeded in the observation of the chemical element 114, one of the heaviest elements created until now. The production of element 114 is very difficult and requires dedicated particle accelerators. So far, this f more

• Elemental bones

  Exposure to chemical pollutants is of growing concern to regulators, health workers, and environmentalist groups alike. Now, researchers in the US and Russia have demonstrated that samples of human bone can act as a biological marker for dozens of metals and toxic elements across the periodic table. more

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• Efficient Transfection of Neuroblastoma Cell Lines Using FuGENE® HD Transfection Reagent
  

  Efficient transfection of cells is a prerequisite for genetic manipulation. However, many cell lines remain hard to transfect with established protocols, hampering consecutive analysis. Here, we compare three different methods (FuGENE® HD Transfection Reagent, transfection reagent L, and calcium phosphate) for the transfection of neuroblastoma cell lines. We show that FuGENE® HD Transfection Reagent is able to transfect all of these cell lines with high efficiency for further analysis. more

• Optimization of Transfection Conditions for the Human HL-60 Promyelocytic Leukemic T-Cell Line Using FuGENE® HD Transfection Reagent
  

  Transfection is commonly performed to introduce many different types of molecules (plasmid DNA, RNA, siRNA, miRNA) into eukaryotic cell lines. Once inside, these molecules can be expressed in a transient or a stable fashion. In the case of transient transfection, molecules do not integrate into the host cell genome and are only expressed for approximately 24 hours - 96 hours post-transfection. more

• Fugene® HD Transfection Reagent: Superior Performance for Challenging Expression Studies
  

  The ability to efficiently introduce foreign molecules into cells constitutes an important tool in any laboratory and facilitates studies of gene regulation and protein function. A plethora of transfection methods have been developed for transfer of DNA into mammalian and animal cells. Those methods either utilize the physicochemical properties of the vector-carrier of the DNA or use mammalian viruses. In the first group of methods, some classic techniques are well-known, such as: calcium phosphate, DEAE-dextran, electroporation liposome-mediated delivery, direct microinjection/biolistic particle delivery, and non-liposomal multi-component reagents. Viral vector systems use both DNA (Adeno-) and RNA (Retro-, Lenti-) viruses. more

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