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  • siRNA Silencing of Lamin A and Quantification of the Knockdown Effect via qPCR

    Lamins are structural proteins which form a thin fibrous layer called the nuclear lamina. Currently, three types of lamins are known in mammalian cells: lamin A, B, and C. Lamins may be involved in DNA replication, chromatin organization, differentiation, nuclear structural support, nuclear envelope reassembly, and several other processes. Mutations of lamin A can cause muscular dystrophy, dilated cardiomyopathy, familiar partial lipodystrophy [3], and other disorders. For the detailed analysis of the different functions of this more than 70 kDa large protein, the expression should be blocked. more

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