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  • A look behind Dalí’s paintings

    A collaboration of scientists, conservators and curators has developed a technique for assessing the condition of works of art without the need to remove samples from them, and used it to analyse a valuable collection of paintings by the enigmatic artist Salvador Dalí. Paintings undergo little mecha more

  • Pfeiffer Vacuum: Q3 the best quarter in the company’s history

    Pfeiffer Vacuum closed the month of September with the best quarter ever in its corporate history. This also produced above-average developments for the first nine months. All metrics posted significant, double-digit growth rates. Pfeiffer Vacuum Chief Executive Officer Manfred Bender had this to s more

  • Sniffing out shoe bombs: A new and simple sensor for explosive chemicals

    University of Illinois chemists have developed a simple sensor to detect an explosive used in shoe bombs. It could lead to inexpensive, easy-to-use devices for luggage and passenger screening at airports and elsewhere. Triacetone triperoxide (TATP) is a high-powered explosive that in recent years h more

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  • Quantitative Detection of Legionella pneumophila in Water Samples: Assay Transfer to the LightCycler® 480 Real-Time PCR System

    An assay to detect and quantify Legionella spp. in water samples was successfully transferred from a capillary-based LightCycler® Instrument to the novel multiwell plate-based LightCycler® 480 System. A previously described protocol was easily adapted by modifying the fluorescent label of the detection probe to match the filter set of the LightCycler® 480 Instrument. While reproducibility and analytical sensitivity of the assay were found to be comparable on both systems, the novel plate-based instrument allowed for higher sample throughput and reduced assay time per sample. more

  • Molecular Probes® NanoOrange® Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

    The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three labelled samples can be loaded on a single gel for comparison. Assay methods for determining protein quantitation include absorbance at 280 nm(1), the Bradford assay(2), Lowry assay(3), BCA method(4) and more sensitive assays such as Fluoroprofile® (Sigma-Aldrich) and NanoOrange® (Molecular Probes®)5(). more

  • LightCycler® 480 System: High-throughput Gene Expression and Genotyping Analysis - A Performance Study

    The LightCycler® 480 System is a novel platform for rapid gene expression and melting curve-based genotyping for medium- and high-throughput. In order to assess the performance and ease-of-use of the complete LightCycler® 480 System (instrument, software, and reagents) in an academic laboratory setting we scheduled a high-throughput project including gene expression (39 targets in 88 samples = 3,400 expression values) as well as genotyping applications (3,808 genotypes; 14 SNPs in 288 samples). The project, involving five technicians and two graduate students, was accomplished within 15 working days. more

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