Topic Acid

Pfeiffer Vacuum closed the month of September with the best quarter ever in its corporate history. This also produced above-average developments for the first nine months. All metrics posted significant, double-digit growth rates. Pfeiffer Vacuum Chief Executive Officer Manfred Bender had this to s more

University of Illinois chemists have developed a simple sensor to detect an explosive used in shoe bombs. It could lead to inexpensive, easy-to-use devices for luggage and passenger screening at airports and elsewhere. Triacetone triperoxide (TATP) is a high-powered explosive that in recent years h more

For the first time, baseclick GmbH, a start-up company spun off from BASF SE and Ludwig-Maximilians-Universität Munich, is making its newly developed process for labeling nucleic acids (DNA and RNA) available to customers. The company recently signed licensing agreements for this technology with thr more

The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three labelled samples can be loaded on a single gel for comparison. Assay methods for determining protein quantitation include absorbance at 280 nm(1), the Bradford assay(2), Lowry assay(3), BCA method(4) and more sensitive assays such as Fluoroprofile® (Sigma-Aldrich) and NanoOrange® (Molecular Probes®)5(). more

The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or affinity chromatography or two-dimensional electrophoresis must be proteolytically cleaved in the course of sample preparation for identification and characterization. A reproducible cleavage pattern of digested proteins is a prerequisite for the unambiguous identification of these proteins by mass spectrometry (MS). more

Manual nucleic acid isolation with High Pure products is long approved by the scientific community. The product portfolio includes kits for isolation of genomic RNA and DNA, plasmid DNA as well as purification of reaction products. Key goal for all products is to offer optimal solutions suited for many different sample materials in order to reduce the number of different nucleic acid isolation kits necessary in modern molecular biology labs. more