analytica-world.com Articles

High sensitive detection of follistatin by Imperacer�: an initial study on the way to analytical gene-doping tests

For the understanding of interactions in the muscle-growth directing myostatin-pathway and therefore also for the establishment of high sensitive detection strategies for gene-doping, detailed studies of protein concentration levels in biological ...

Molecular Probes� NanoOrange� Assay Performed on BMG LABTECH FLUOstar OPTIMA Microplate Reader

The field of proteomics has expanded dramatically in recent years with research on a whole host of organisms. Techniques such as two dimensional difference gel electrophoresis (2D DIGE) require the accurate quantitation of protein, as up to three ...

Nature's Ingenuity Shows the Way

Up to 80 percent of our experience of our visual environment takes place via our visual perception. Without spatial vision, we would hardly be able to stay oriented. In recent decades, the neurosciences have gained many insights into the complex ...

Bradford Assay Performed on BMG LABTECH�s FLUOstar Omega with new Evaluation Software

Determining the protein concentration of samples is a necessary and often used method in biochemistry. Different colorimetric protein assays have been developed. The most commonly used methods are the Bradford assay, the Lowry assay and the BCA ...

Sensible and self-monitoring

For the generation of soft water are mostly used ion exchanging devices with generation of sodium chloride since the beginning of the Sixties. The generated soft water quality and soft water quantity are depending thereby on the static and dynamic ...

Efficient Transfection of Primary Human Skeletal Myoblasts Using FuGENE� HD Transfection Reagent

Transfection of cells is one of the main techniques used to influence gene expression. Most primary cells and human skeletal myoblasts (SkMC) in particular are very difficult to transfect, whereas for cell lines such as C2C12, many suitable ...

Enzymatic Protein Digest for Mass-spectrometric Analysis

The main focus in proteomic studies is on the identification of proteins in given biological samples. Proteins isolated and separated from a given sample (e.g., whole cell lysates, blood or tissue, protein complexes) by immunoprecipitation or ...

Real-Time Multiplex PCR of five Different DNA Targets Using the LightCycler� 480 System

One of the most interesting aspects of real-time PCR based on detection of fluorophoric-labeled oligonucleo�tides, such as Hydrolysis probes, and Molecular Beacons, is the possibility of being able to detect conveniently multiple targets in the same ...

High Pure PCR Cleanup Micro Kit - Novel Flexibility for Nucleic Acid Purification

Manual nucleic acid isolation with High Pure products is long approved by the scientific community. The product portfolio includes kits for isolation of genomic RNA and DNA, plasmid DNA as well as purification of reaction products. Key goal for all ...

Mutation Scanning of the Cytidine Deaminase Gene by High-Resolution Melting Curve Analysis Using the LightCycler� 480 System

Alexandre Evrard1,2, Caroline Raynal1,2, Jean-Christophe Boyer2, Lionel Le Gallic2, and Serge Lumbroso1,2 1Institut de G�nomique Fonctionnelle, D�partement d'Oncologie Mol�culaire et Cellulaire, Montpellier, France, 2Laboratoire de Biochimie, ...

Genotypes in Smokers: Correlations with Smoking Behavior

Smoking behavior is influenced by both genetic and environmental factors. Moreover, smoking initiation and smoking persistence have a heritability of at least 50%. A large body of work has been dedicated to associating multiple genetic markers of ...

Metagenomics Analysis Using the Genome Sequencer FLX System

The Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science is a versatile sequencing platform suitable for a wide range of applications, including de novo sequencing and assembly of genomic DNA, transcriptome sequencing, small ...

Experimental Workflow for Fast and Accurate Genotyping of Scrapie-resistant/sensitive Sheep

Several scientific studies support the claim that natural scrapie is associated with polymorphisms at three codons within the sheep prion protein (PrP) gene. Genotyping of these codons (codon 136 [alanine, valine], 154 [arginine, histidine], and 171 ...

The Universal ProbeLibrary - a Versatile Tool for Quantitative Expression Analysis in the Zebrafish

The teleost zebrafish is a widely used model organism for the study of developmental and disease-related processes in vertebrates, including humans. Understanding these processes at a molecular and cellular level frequently requires the estimation ...

Real-Time PCR Quantification of Plant miRNAs Using Universal ProbeLibrary Technology

MicroRNAs (miRNAs) are naturally occurring, highly conserved families of transcripts (18-25 nt in length) that are processed from larger hairpin precursors. Both plant and animal genomes encode miRNAs, and to date, there are over 4000 mature miRNA ...

Real-Time PCR Quality Control for Gene Expression

Quantitative real-time PCR (qPCR) has become the de facto standard for nucleic acid quantification. This achievement is due in large part to its sensitivity, specificity, accuracy, large dynamic range of linear quantification, and its speed. The ...

New Application for the LightCycler� 480 System: Gene Scanning by High-Resolution Melting

High-resolution melting (HRM) is a novel, homogeneous, post-PCR method, enabling genomic researchers to analyze genetic variations (SNPs, mutations, methylations) in PCR amplicons. The most important high-resolution melting application is gene ...

Enabling Cancer Research with FuGENE� HD Transfection Reagent

A major problem in some areas of cancer research is the difficulty of transfecting a significant number of the cells of interest, while minimizing the cytotoxic or other off-target effects of the transfection event itself. It is well-known that the ...

The New Genome Sequencer FLX System

In 2005, the Genome Sequencer 20 System was introduced into the global life science market. Based on the revolutionary 454 sequencing technology, this system is able to generate hundreds of thousands of sequence reads in a few hours at a fraction of ...

Quantification of Zea mays mRNAs by Real-Time PCR Using the Universal ProbeLibrary

The Universal ProbeLibrary (UPL) is a detection system for real-time PCR based on locked nucleic acid (LNA) probes. We tested the efficiency of the UPL for the quantification of maize transcripts on two detection platforms. The results indicate that ...